Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23812 | 3' | -58.3 | NC_005261.1 | + | 37004 | 0.68 | 0.683166 |
Target: 5'- cCGCGGGCUgCGCGGGGccaGUUUgCGg -3' miRNA: 3'- -GCGCUCGAgGCGCUCCaugCAGAgGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 57294 | 0.66 | 0.831567 |
Target: 5'- uCGCGAGCagCCGCGcg--ACGUCcucgUCCAu -3' miRNA: 3'- -GCGCUCGa-GGCGCuccaUGCAG----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 48794 | 0.67 | 0.732331 |
Target: 5'- uCGCcGGCUCCGUGAGGccgUGCGcggCgUCCGc -3' miRNA: 3'- -GCGcUCGAGGCGCUCC---AUGCa--G-AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 86284 | 0.67 | 0.732331 |
Target: 5'- gCGCGAGCacagcCCGCaGGGcACGUCgcccUCCAg -3' miRNA: 3'- -GCGCUCGa----GGCGcUCCaUGCAG----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 13100 | 0.67 | 0.732331 |
Target: 5'- gGCGGGCUCgGCucGGGG-GCGUCggcggCCGc -3' miRNA: 3'- gCGCUCGAGgCG--CUCCaUGCAGa----GGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 123250 | 0.67 | 0.729429 |
Target: 5'- gCGCGGGCcCCGCccaucgcccgugacGAGGUGCGgguggUCCGc -3' miRNA: 3'- -GCGCUCGaGGCG--------------CUCCAUGCag---AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 70926 | 0.68 | 0.722631 |
Target: 5'- gCGCGAGCUCCaGCGccuGcGCGUCcgCCGg -3' miRNA: 3'- -GCGCUCGAGG-CGCuc-CaUGCAGa-GGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 121656 | 0.68 | 0.711875 |
Target: 5'- gCGCGGGCugUCCGCGucggcguAGGUguagGCGccCUCCAg -3' miRNA: 3'- -GCGCUCG--AGGCGC-------UCCA----UGCa-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 120312 | 0.68 | 0.703014 |
Target: 5'- gGCGAGgCugUCUGCGAGGcgcgcUGCGUCcCCGa -3' miRNA: 3'- gCGCUC-G--AGGCGCUCC-----AUGCAGaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 104171 | 0.68 | 0.692122 |
Target: 5'- cCGCGGGCgCCGCGcgcGGGU-CGUCgggcgggacggcgUCCAg -3' miRNA: 3'- -GCGCUCGaGGCGC---UCCAuGCAG-------------AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 75081 | 0.68 | 0.683166 |
Target: 5'- gCGgGGGCUCgGCGGGcauCGUCUUCGg -3' miRNA: 3'- -GCgCUCGAGgCGCUCcauGCAGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 109326 | 0.79 | 0.18981 |
Target: 5'- cCGCGAGCgcggCCGCGGGGUcggcggccGCGUCgccgUCCAg -3' miRNA: 3'- -GCGCUCGa---GGCGCUCCA--------UGCAG----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 81911 | 0.68 | 0.677176 |
Target: 5'- aCGCGgccAGCUCCuccuUGAGGUGCGggagcaggcccacguUCUCCAc -3' miRNA: 3'- -GCGC---UCGAGGc---GCUCCAUGC---------------AGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 39232 | 0.68 | 0.673176 |
Target: 5'- uCGCGcGCgCCgGCGAGGUcuccggcgAgGUCUCCAg -3' miRNA: 3'- -GCGCuCGaGG-CGCUCCA--------UgCAGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 60360 | 0.69 | 0.660142 |
Target: 5'- gCGCGccaGGCagugcuuggucucgUUgGCGAGGUGCGcCUCCAu -3' miRNA: 3'- -GCGC---UCG--------------AGgCGCUCCAUGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 98676 | 0.69 | 0.653107 |
Target: 5'- gGCGGGCUCCGCcggggccgGAGGcGCGg--CCGg -3' miRNA: 3'- gCGCUCGAGGCG--------CUCCaUGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 135747 | 0.69 | 0.643045 |
Target: 5'- gGCGcGCcucagCCGCGcGGUGCG-CUCCGg -3' miRNA: 3'- gCGCuCGa----GGCGCuCCAUGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 76648 | 0.69 | 0.612838 |
Target: 5'- aCGCGAGCgcCUGCGAGGU-CGgcgCCGu -3' miRNA: 3'- -GCGCUCGa-GGCGCUCCAuGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 44879 | 0.7 | 0.572788 |
Target: 5'- cCGCGAGCgCCGCGGGG-AUGg--CCGg -3' miRNA: 3'- -GCGCUCGaGGCGCUCCaUGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 86198 | 0.7 | 0.572788 |
Target: 5'- aGCGGGCUCCGCGucGGcGCGcCgaaCCGg -3' miRNA: 3'- gCGCUCGAGGCGCu-CCaUGCaGa--GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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