Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
23813 | 5' | -59.4 | NC_005261.1 | + | 132641 | 0.73 | 0.348167 |
Target: 5'- gACGCGGGcgcGGCGgcccuagcgGCCGCGugUGGGu -3' miRNA: 3'- aUGCGCCC---CUGCa--------UGGCGCugACCUu -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 88146 | 0.74 | 0.304216 |
Target: 5'- cGCGCGGGGGCGccccgGCCGCGcaGCcGGGc -3' miRNA: 3'- aUGCGCCCCUGCa----UGGCGC--UGaCCUu -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 68087 | 0.75 | 0.264653 |
Target: 5'- -cCGCGGGGGCGgggagGCCGUgaaguucgGGCUGGAGc -3' miRNA: 3'- auGCGCCCCUGCa----UGGCG--------CUGACCUU- -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 68852 | 0.77 | 0.188582 |
Target: 5'- -cCGCGGGGACGcccucUGCCGCGGCUGu-- -3' miRNA: 3'- auGCGCCCCUGC-----AUGGCGCUGACcuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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