Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23813 | 5' | -59.4 | NC_005261.1 | + | 100166 | 0.66 | 0.734301 |
Target: 5'- aGCGCGcccaGGGACGcaacGCCGUGGCggcgGGGc -3' miRNA: 3'- aUGCGC----CCCUGCa---UGGCGCUGa---CCUu -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 110813 | 0.66 | 0.734301 |
Target: 5'- gAgGCGGGGGCGgacgggGCCGCGGac-GAGu -3' miRNA: 3'- aUgCGCCCCUGCa-----UGGCGCUgacCUU- -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 127747 | 0.66 | 0.734301 |
Target: 5'- gGCgGCGGGaGCGgGCCGCGGC-GGGc -3' miRNA: 3'- aUG-CGCCCcUGCaUGGCGCUGaCCUu -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 43786 | 0.66 | 0.743965 |
Target: 5'- cGCGCGGaGGGC--GCCGCGGgUGaGGGg -3' miRNA: 3'- aUGCGCC-CCUGcaUGGCGCUgAC-CUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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