Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23813 | 5' | -59.4 | NC_005261.1 | + | 8842 | 0.68 | 0.583748 |
Target: 5'- gGgGCGGGGACagGCCGUGGgaGGGg -3' miRNA: 3'- aUgCGCCCCUGcaUGGCGCUgaCCUu -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 8375 | 0.66 | 0.704821 |
Target: 5'- -uUGUGGGGAgGgcccccccaaGCCGgGGCUGGGAu -3' miRNA: 3'- auGCGCCCCUgCa---------UGGCgCUGACCUU- -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 2646 | 0.67 | 0.644467 |
Target: 5'- cACGCGGGcgggcagcGGCGgcucccGCCGCG-CUGGGGa -3' miRNA: 3'- aUGCGCCC--------CUGCa-----UGGCGCuGACCUU- -5' |
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23813 | 5' | -59.4 | NC_005261.1 | + | 445 | 0.72 | 0.396468 |
Target: 5'- cGgGaCGGGGACGgggGCCGCGACggccggcgGGAu -3' miRNA: 3'- aUgC-GCCCCUGCa--UGGCGCUGa-------CCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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