Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2382 | 3' | -59.5 | NC_001416.1 | + | 5730 | 0.66 | 0.461472 |
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Target: 5'- cCUGCaGCCAG-CGGUGCCGGu--GCc -3' miRNA: 3'- -GACGcUGGUCaGUCACGGCCugcCGa -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 5208 | 0.66 | 0.461472 |
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Target: 5'- gCUGCaGugUacagcGGUCAGgaggccauugaUGCCGGACuGGCUg -3' miRNA: 3'- -GACG-CugG-----UCAGUC-----------ACGGCCUG-CCGA- -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 20086 | 0.66 | 0.451714 |
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Target: 5'- -cGCGccGCCAG-CAcGUccGCCGGACaGGCUg -3' miRNA: 3'- gaCGC--UGGUCaGU-CA--CGGCCUG-CCGA- -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 9147 | 0.66 | 0.44207 |
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Target: 5'- aCUGCGACCGGgCAGgggcagaaaucUGCCGGAgauacCaGCUu -3' miRNA: 3'- -GACGCUGGUCaGUC-----------ACGGCCU-----GcCGA- -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 17481 | 0.67 | 0.404699 |
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Target: 5'- -aGCGGCUGGUCAGcacgGCCcGGACGaCg -3' miRNA: 3'- gaCGCUGGUCAGUCa---CGG-CCUGCcGa -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 4740 | 0.68 | 0.3283 |
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Target: 5'- -gGCuGACCAG-C-GUGCCGGAcacCGGCa -3' miRNA: 3'- gaCG-CUGGUCaGuCACGGCCU---GCCGa -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 14948 | 0.69 | 0.305317 |
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Target: 5'- -aGCuGugUGGcCAGUGcCCGGAUGGCUu -3' miRNA: 3'- gaCG-CugGUCaGUCAC-GGCCUGCCGA- -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 20217 | 0.77 | 0.084832 |
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Target: 5'- -gGCGGCCAc-CAGUGCCGGuGCGGCg -3' miRNA: 3'- gaCGCUGGUcaGUCACGGCC-UGCCGa -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 20892 | 0.79 | 0.058663 |
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Target: 5'- -gGCGuuCCGGUCAGUGCCGGACuguccagugGGCUu -3' miRNA: 3'- gaCGCu-GGUCAGUCACGGCCUG---------CCGA- -5' |
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| 2382 | 3' | -59.5 | NC_001416.1 | + | 16408 | 1.07 | 0.000442 |
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Target: 5'- aCUGCGACCAGUCAGUGCCGGACGGCUu -3' miRNA: 3'- -GACGCUGGUCAGUCACGGCCUGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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