Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23824 | 3' | -59.1 | NC_005261.1 | + | 19932 | 0.66 | 0.788935 |
Target: 5'- cGCCCUCgcuagcguCCUGgGCGGGGGUggggcggCCUCGg -3' miRNA: 3'- -CGGGAG--------GGGUgUGUCCCUAga-----GGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 19794 | 0.66 | 0.788935 |
Target: 5'- gGCCCUCCgaGC-CGGGGGUCagCCgcCGg -3' miRNA: 3'- -CGGGAGGggUGuGUCCCUAGa-GGa-GC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 11623 | 0.66 | 0.779916 |
Target: 5'- cGCCCUcccgcCCCCACGCGGcGccC-CCUCGa -3' miRNA: 3'- -CGGGA-----GGGGUGUGUCcCuaGaGGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 87371 | 0.66 | 0.77077 |
Target: 5'- cGCCCa--CCGCGCGGcaGAUgUCCUCGg -3' miRNA: 3'- -CGGGaggGGUGUGUCc-CUAgAGGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 84929 | 0.66 | 0.77077 |
Target: 5'- gGCCUcggCCUCGCGCAGcac-CUCCUCGg -3' miRNA: 3'- -CGGGa--GGGGUGUGUCccuaGAGGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 2296 | 0.66 | 0.77077 |
Target: 5'- aGCgCCUCCCCGgGCAGcGGGUCg----- -3' miRNA: 3'- -CG-GGAGGGGUgUGUC-CCUAGaggagc -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 79021 | 0.67 | 0.742654 |
Target: 5'- cCCCUCCUCGCcCGGGGcgCccgcgcCCUUGg -3' miRNA: 3'- cGGGAGGGGUGuGUCCCuaGa-----GGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 14779 | 0.67 | 0.733085 |
Target: 5'- gGCCg-CCCCGCGCAGGuGccgCUCC-CGc -3' miRNA: 3'- -CGGgaGGGGUGUGUCC-Cua-GAGGaGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 82234 | 0.67 | 0.723431 |
Target: 5'- aGCaCgUCCCCguagacgcucacGCGCAGGG-UCUUCUCc -3' miRNA: 3'- -CG-GgAGGGG------------UGUGUCCCuAGAGGAGc -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 58828 | 0.67 | 0.713703 |
Target: 5'- cGUCCUCCaggcacCCGCGCuGGG-UCUCC-CGc -3' miRNA: 3'- -CGGGAGG------GGUGUGuCCCuAGAGGaGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 95889 | 0.67 | 0.694054 |
Target: 5'- cGCCCUCuuaacgcgcgcgCCCGCGCGGcGcgCUCaCUCGc -3' miRNA: 3'- -CGGGAG------------GGGUGUGUCcCuaGAG-GAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 13820 | 0.68 | 0.68415 |
Target: 5'- gGCUCgaCCCGCAgcguCAGGGucgacugcGUCUCCUCGu -3' miRNA: 3'- -CGGGagGGGUGU----GUCCC--------UAGAGGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 132317 | 0.68 | 0.674206 |
Target: 5'- cGCCCcaUCCCC-CGCGGGGAcCUgCaCGg -3' miRNA: 3'- -CGGG--AGGGGuGUGUCCCUaGAgGaGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 9458 | 0.68 | 0.664228 |
Target: 5'- cCCCUCCCCGCAgAGGGGggaggggCUUGc -3' miRNA: 3'- cGGGAGGGGUGUgUCCCUagag---GAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 16322 | 0.68 | 0.664228 |
Target: 5'- gGCCCUCCCCcuCGCcgccGGUCUCCUg- -3' miRNA: 3'- -CGGGAGGGGu-GUGucc-CUAGAGGAgc -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 132720 | 0.68 | 0.658229 |
Target: 5'- cGCCCUaccucauugcggcggCCCCGCACucaAGGGcAUCgcgcgCCUUGu -3' miRNA: 3'- -CGGGA---------------GGGGUGUG---UCCC-UAGa----GGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 127297 | 0.68 | 0.654225 |
Target: 5'- aGCCCUCCCCGCcggcugGCGGuGGggC-CCUg- -3' miRNA: 3'- -CGGGAGGGGUG------UGUC-CCuaGaGGAgc -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 77871 | 0.68 | 0.648215 |
Target: 5'- cGCCCUCUCCAgGCucgcguccgccGUCUCCUCGc -3' miRNA: 3'- -CGGGAGGGGUgUGuccc-------UAGAGGAGC- -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 59751 | 0.69 | 0.624147 |
Target: 5'- cGCCCUCCaCCAgGCAGGc--CUCCa-- -3' miRNA: 3'- -CGGGAGG-GGUgUGUCCcuaGAGGagc -5' |
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23824 | 3' | -59.1 | NC_005261.1 | + | 102453 | 0.69 | 0.624147 |
Target: 5'- cGCCCggCCUCggcGCGCAGgcgccGGAcCUCCUCGg -3' miRNA: 3'- -CGGGa-GGGG---UGUGUC-----CCUaGAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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