Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23824 | 5' | -56.3 | NC_005261.1 | + | 137115 | 0.69 | 0.747038 |
Target: 5'- cCCGcgcGGCGGCGUuuUGUGGAaggagcGAGGGAg -3' miRNA: 3'- aGGCu--CCGCUGCG--ACACCUa-----CUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 77196 | 0.66 | 0.86117 |
Target: 5'- aUCUGcAGGCGAgcgcgguguuccCGCUGgccGGggGGGAGGa -3' miRNA: 3'- -AGGC-UCCGCU------------GCGACa--CCuaCUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 74851 | 0.66 | 0.896654 |
Target: 5'- --gGAGGCGGCGCgccgGGcgGAG-GAg -3' miRNA: 3'- aggCUCCGCUGCGaca-CCuaCUCuCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 73154 | 0.68 | 0.811853 |
Target: 5'- gCCGccAGGCGGCGUUGUugcucggccgGGggGAGGGu -3' miRNA: 3'- aGGC--UCCGCUGCGACA----------CCuaCUCUCu -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 70181 | 0.69 | 0.747038 |
Target: 5'- -gCGGGaGCGGcCGCUGUGGAggcGGGGGu -3' miRNA: 3'- agGCUC-CGCU-GCGACACCUa--CUCUCu -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 68586 | 0.67 | 0.848689 |
Target: 5'- gCCGAGGCGGgcCGCg--GGAgcggggccgacggcgUGGGGGAg -3' miRNA: 3'- aGGCUCCGCU--GCGacaCCU---------------ACUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 62741 | 0.66 | 0.868703 |
Target: 5'- uUCUGgaGGGCGGCcaggucGCUGUGG--GAGAGGu -3' miRNA: 3'- -AGGC--UCCGCUG------CGACACCuaCUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 50991 | 0.68 | 0.794022 |
Target: 5'- gCCGAcGGCGGCGC-GUGGGcgcggcGGGAGc -3' miRNA: 3'- aGGCU-CCGCUGCGaCACCUa-----CUCUCu -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 43777 | 0.69 | 0.737301 |
Target: 5'- -gUGGGGCGGCGCgcggagGgcgccgcGGGUGAGGGGg -3' miRNA: 3'- agGCUCCGCUGCGa-----Ca------CCUACUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 37375 | 0.79 | 0.238976 |
Target: 5'- cCCGAGGCGGCGCUGUGGuacgcGGAc -3' miRNA: 3'- aGGCUCCGCUGCGACACCuacucUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 35249 | 0.66 | 0.890003 |
Target: 5'- --aGAGGCGGCG----GGAUGGGAGGc -3' miRNA: 3'- aggCUCCGCUGCgacaCCUACUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 33828 | 0.66 | 0.889325 |
Target: 5'- gCCGGGGggcgcgggcccccUGGCGCUGggcgGGccGGGAGGg -3' miRNA: 3'- aGGCUCC-------------GCUGCGACa---CCuaCUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 30377 | 0.68 | 0.775605 |
Target: 5'- gCCGGGcgccGCGGCGCggGUGGAgguGGAGGa -3' miRNA: 3'- aGGCUC----CGCUGCGa-CACCUac-UCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 24121 | 0.68 | 0.803015 |
Target: 5'- cCCGAGGCGGgGgUGggggcUGGGUGGGcGGGg -3' miRNA: 3'- aGGCUCCGCUgCgAC-----ACCUACUC-UCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 23624 | 0.67 | 0.836529 |
Target: 5'- gCCGGGGCGcggcgggggucggGCgGCUGgaagGGAgagGGGAGGa -3' miRNA: 3'- aGGCUCCGC-------------UG-CGACa---CCUa--CUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 22081 | 0.7 | 0.667087 |
Target: 5'- cUCGAGGgGGCGCcgcGUGGGggcGGGAGGg -3' miRNA: 3'- aGGCUCCgCUGCGa--CACCUa--CUCUCU- -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 14369 | 0.66 | 0.86117 |
Target: 5'- gCCGGGGCGGCGCgGUGGc------- -3' miRNA: 3'- aGGCUCCGCUGCGaCACCuacucucu -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 13702 | 0.68 | 0.811853 |
Target: 5'- -gCGGGGCGG-GCUGggggcggGGGUGGGGGu -3' miRNA: 3'- agGCUCCGCUgCGACa------CCUACUCUCu -5' |
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23824 | 5' | -56.3 | NC_005261.1 | + | 7979 | 0.75 | 0.38753 |
Target: 5'- gCCGAGGCGGCcCgGUGGGaGAGGGGg -3' miRNA: 3'- aGGCUCCGCUGcGaCACCUaCUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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