Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23826 | 3' | -53.9 | NC_005261.1 | + | 70162 | 0.66 | 0.949996 |
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Target: 5'- uGGGGAACAGCagCaGGgCGCGGgagCGg -3' miRNA: 3'- -CUCCUUGUCGaaGaCCaGCGUCa--GCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 71284 | 0.66 | 0.945585 |
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Target: 5'- ---cAGCAGCUUgaGGUgGUAGUCGa -3' miRNA: 3'- cuccUUGUCGAAgaCCAgCGUCAGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 107752 | 0.66 | 0.925436 |
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Target: 5'- gGGGGGGCcacgccccAGCgcucgCUGGcgCGCGGUCGa -3' miRNA: 3'- -CUCCUUG--------UCGaa---GACCa-GCGUCAGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 25414 | 0.67 | 0.913846 |
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Target: 5'- uGAGGGGCAGCuUUCUGGUCcCcaaaUCGa -3' miRNA: 3'- -CUCCUUGUCG-AAGACCAGcGuc--AGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 84122 | 0.68 | 0.887672 |
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Target: 5'- aGGGGGGCAGCgggCggGGUCGCuGgCGg -3' miRNA: 3'- -CUCCUUGUCGaa-Ga-CCAGCGuCaGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 17746 | 0.68 | 0.887672 |
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Target: 5'- gGAGcGGGCGGaCUUCUGGg-GCGGUgGUg -3' miRNA: 3'- -CUC-CUUGUC-GAAGACCagCGUCAgCA- -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 34809 | 0.68 | 0.873138 |
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Target: 5'- gGAGGAcgaGGaCUcCUGGUCGgAGUCGg -3' miRNA: 3'- -CUCCUug-UC-GAaGACCAGCgUCAGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 85072 | 0.68 | 0.873138 |
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Target: 5'- --aGAACuGCUUgUGGUCGCAGUa-- -3' miRNA: 3'- cucCUUGuCGAAgACCAGCGUCAgca -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 98935 | 0.68 | 0.873138 |
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Target: 5'- cGGGGGGCAGCgcgUCc--UCGUAGUCGa -3' miRNA: 3'- -CUCCUUGUCGa--AGaccAGCGUCAGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 116153 | 0.68 | 0.865525 |
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Target: 5'- aGAGGaAGCAGCgcgcGGUCGCGGcCGc -3' miRNA: 3'- -CUCC-UUGUCGaagaCCAGCGUCaGCa -5' |
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| 23826 | 3' | -53.9 | NC_005261.1 | + | 3979 | 0.97 | 0.022151 |
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Target: 5'- gGAGGAACAGCUUC-GGUCGCAGUCGUa -3' miRNA: 3'- -CUCCUUGUCGAAGaCCAGCGUCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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