Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23827 | 5' | -58.6 | NC_005261.1 | + | 35075 | 0.68 | 0.650436 |
Target: 5'- cUCCGCGCCCC-CUCGCccUCggagCCg-- -3' miRNA: 3'- -AGGCGCGGGGaGAGUGauAGa---GGgaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 26406 | 0.66 | 0.778891 |
Target: 5'- cUCGCGCuCUCUCUCccccCUucUCUCCCUc -3' miRNA: 3'- aGGCGCG-GGGAGAGu---GAu-AGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 94456 | 0.66 | 0.769543 |
Target: 5'- gUCCGCGucCCCCUC-CGCgucggcGUCgUCCCg- -3' miRNA: 3'- -AGGCGC--GGGGAGaGUGa-----UAG-AGGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 111809 | 0.66 | 0.750488 |
Target: 5'- cUCGCGUCggCCgagCUCGCgcuUCUCCCUg -3' miRNA: 3'- aGGCGCGG--GGa--GAGUGau-AGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 6853 | 0.66 | 0.7408 |
Target: 5'- cCCcCGCCCCaUUCACg--CUCCCg- -3' miRNA: 3'- aGGcGCGGGGaGAGUGauaGAGGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 51591 | 0.66 | 0.730034 |
Target: 5'- gCCGUGCUCCUCcugaCGCUGUCUgagcucgUCCUg -3' miRNA: 3'- aGGCGCGGGGAGa---GUGAUAGA-------GGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 22325 | 0.67 | 0.721148 |
Target: 5'- cCCGCGCCCgCcCaCACUccCUCCCUc -3' miRNA: 3'- aGGCGCGGG-GaGaGUGAuaGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 33205 | 0.67 | 0.720157 |
Target: 5'- cCCGCGCccuccccCCCUCUCcCUccccCUCCCUc -3' miRNA: 3'- aGGCGCG-------GGGAGAGuGAua--GAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 133112 | 0.67 | 0.711203 |
Target: 5'- aCCGCGCggCCUUC-CACggcgugcgcGUCUCCCUg -3' miRNA: 3'- aGGCGCG--GGGAGaGUGa--------UAGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 58819 | 0.67 | 0.701191 |
Target: 5'- gCCGCGCCacguCCUCcaggcacccgCGCUggGUCUCCCg- -3' miRNA: 3'- aGGCGCGG----GGAGa---------GUGA--UAGAGGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 7386 | 1.05 | 0.002437 |
Target: 5'- uUCCGCGCCCCUCUCACUAUCUCCCUUc -3' miRNA: 3'- -AGGCGCGGGGAGAGUGAUAGAGGGAA- -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 10516 | 0.68 | 0.650436 |
Target: 5'- gCCGCugcggGCCCCgcggcgcgCUCGCUGUCgccCCCg- -3' miRNA: 3'- aGGCG-----CGGGGa-------GAGUGAUAGa--GGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 120334 | 0.68 | 0.640207 |
Target: 5'- gCUGCGUCCC-CgagCGC-GUCUCCCUg -3' miRNA: 3'- aGGCGCGGGGaGa--GUGaUAGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 50360 | 0.68 | 0.609514 |
Target: 5'- gCCGCGCCCgUCaUCACguagCUCCg-- -3' miRNA: 3'- aGGCGCGGGgAG-AGUGaua-GAGGgaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 100084 | 0.7 | 0.53881 |
Target: 5'- gCCGCGCCCCUCgccgccgcCGCUGcCgccgCCCa- -3' miRNA: 3'- aGGCGCGGGGAGa-------GUGAUaGa---GGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 28315 | 0.71 | 0.480532 |
Target: 5'- gCgGCGCCCgcgCUCUCGC--UCUCCCUc -3' miRNA: 3'- aGgCGCGGG---GAGAGUGauAGAGGGAa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 78792 | 0.71 | 0.471112 |
Target: 5'- gCCGCagggGCuCCCUCUCACgg-CUCCCc- -3' miRNA: 3'- aGGCG----CG-GGGAGAGUGauaGAGGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 91565 | 0.72 | 0.425497 |
Target: 5'- gCCGUGCCCCUCaacaccgacUACUAcuUCUCCCa- -3' miRNA: 3'- aGGCGCGGGGAGa--------GUGAU--AGAGGGaa -5' |
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23827 | 5' | -58.6 | NC_005261.1 | + | 22254 | 0.75 | 0.278682 |
Target: 5'- cUCCGCGCCCgCUC-CGCUucuuUUUCCCUc -3' miRNA: 3'- -AGGCGCGGG-GAGaGUGAu---AGAGGGAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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