Results 1 - 20 of 110 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23830 | 5' | -59.3 | NC_005261.1 | + | 17722 | 0.66 | 0.73454 |
Target: 5'- -uGCccGGGAAucGGAGCGgcgcuCGGAGCGgGCg -3' miRNA: 3'- cuCGa-CCCUU--CCUCGC-----GCCUCGUgUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 120428 | 0.66 | 0.73454 |
Target: 5'- uGGGCUGGuGAAcauGCGCGGcGUGCGCc -3' miRNA: 3'- -CUCGACC-CUUccuCGCGCCuCGUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 9126 | 0.66 | 0.73454 |
Target: 5'- -cGCcGGGAcuGGGGGCG-GGuGCAgGCa -3' miRNA: 3'- cuCGaCCCU--UCCUCGCgCCuCGUgUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 98920 | 0.66 | 0.73454 |
Target: 5'- cGGCgGGGucGGcuGCGgGGGGCAgCGCg -3' miRNA: 3'- cUCGaCCCuuCCu-CGCgCCUCGU-GUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 101685 | 0.66 | 0.73454 |
Target: 5'- cGAGCcGGcGAAGcGGGCGUGGucgaAGCGCu- -3' miRNA: 3'- -CUCGaCC-CUUC-CUCGCGCC----UCGUGug -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 21618 | 0.66 | 0.73454 |
Target: 5'- cGAGCUucGGGcGAGGguccccgccccGGCGCGGAcGC-CGCu -3' miRNA: 3'- -CUCGA--CCC-UUCC-----------UCGCGCCU-CGuGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 73206 | 0.66 | 0.73454 |
Target: 5'- uGGCcccGGGcgccucGAGGGGCGCGGuGGC-CGCg -3' miRNA: 3'- cUCGa--CCC------UUCCUCGCGCC-UCGuGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 48028 | 0.66 | 0.724715 |
Target: 5'- aGAGCaGGGcGAGGAGCaCGc-GCGCGCg -3' miRNA: 3'- -CUCGaCCC-UUCCUCGcGCcuCGUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 92140 | 0.66 | 0.724715 |
Target: 5'- cGGGCUGGGccAGGAcCuCGGcgAGCGCGCg -3' miRNA: 3'- -CUCGACCCu-UCCUcGcGCC--UCGUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 58303 | 0.66 | 0.723728 |
Target: 5'- gGGGCgcgGGGAGGGGGCGgcccagcCGcccGAGCAC-Cg -3' miRNA: 3'- -CUCGa--CCCUUCCUCGC-------GC---CUCGUGuG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 56005 | 0.66 | 0.714811 |
Target: 5'- cGGCggGGGcGGGGGCGgGGAagACGCa -3' miRNA: 3'- cUCGa-CCCuUCCUCGCgCCUcgUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 27169 | 0.66 | 0.714811 |
Target: 5'- gGGGCgguaagugGGGAAGGAaaGgGGAGCAaACg -3' miRNA: 3'- -CUCGa-------CCCUUCCUcgCgCCUCGUgUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 68615 | 0.66 | 0.714811 |
Target: 5'- cGGCgugGGGGAGGAuGCGCGcccGCcCGCg -3' miRNA: 3'- cUCGa--CCCUUCCU-CGCGCcu-CGuGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 22094 | 0.66 | 0.704836 |
Target: 5'- -cGCgUGGGGgcGGGAGgGCGGuGUGCGg -3' miRNA: 3'- cuCG-ACCCU--UCCUCgCGCCuCGUGUg -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 72446 | 0.66 | 0.703835 |
Target: 5'- cGGCgGGGGcgcccgcGGcGAGCGCGGcgAGCGCGa -3' miRNA: 3'- cUCGaCCCU-------UC-CUCGCGCC--UCGUGUg -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 44207 | 0.66 | 0.703835 |
Target: 5'- cGAGCUGcaggcGGcccGGGAGCGCGcGcugcuggccgcccAGCGCGCg -3' miRNA: 3'- -CUCGAC-----CCu--UCCUCGCGC-C-------------UCGUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 15969 | 0.66 | 0.698821 |
Target: 5'- cGGGCgGGGGAGG-GCcCGGgccgcucacccgggcAGCGCGCu -3' miRNA: 3'- -CUCGaCCCUUCCuCGcGCC---------------UCGUGUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 11432 | 0.66 | 0.6948 |
Target: 5'- cGGGCgGGGggGGcggcggaagagGGaaaaagaaGCGGAGCGgGCg -3' miRNA: 3'- -CUCGaCCCuuCC-----------UCg-------CGCCUCGUgUG- -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 98356 | 0.66 | 0.6948 |
Target: 5'- gGGGCgGGGguGG-GCGCGGgcucuggcgGGCGCAa -3' miRNA: 3'- -CUCGaCCCuuCCuCGCGCC---------UCGUGUg -5' |
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23830 | 5' | -59.3 | NC_005261.1 | + | 84108 | 0.66 | 0.693793 |
Target: 5'- gGGGCgggaugGGGAGGGGGgcaGCGGGcggggucGCugGCg -3' miRNA: 3'- -CUCGa-----CCCUUCCUCg--CGCCU-------CGugUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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