Results 1 - 20 of 87 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23835 | 5' | -59.3 | NC_005261.1 | + | 96846 | 0.66 | 0.771836 |
Target: 5'- gGCCGGGGucgacgGGGGGCucGGGggGcgcGGGu -3' miRNA: 3'- -UGGCCUUca----CCCCCGcuCCCuuC---UCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 7332 | 0.66 | 0.771836 |
Target: 5'- -aCGGAAaaGGGGGaggaggaGAGaGGGAGAGGg -3' miRNA: 3'- ugGCCUUcaCCCCCg------CUC-CCUUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 25380 | 0.66 | 0.771836 |
Target: 5'- cCCGGcacGGcGcGcGGGuCGGGGGAGGAGAg -3' miRNA: 3'- uGGCCu--UCaC-C-CCC-GCUCCCUUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 68599 | 0.66 | 0.771836 |
Target: 5'- -gCGGGAGcGGGGccgacggcGUGGGGGAGGAu- -3' miRNA: 3'- ugGCCUUCaCCCC--------CGCUCCCUUCUcu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 69925 | 0.66 | 0.762552 |
Target: 5'- aACCgcaGGggGUGGGGugggaGCcGGGGAuagcGGAGGg -3' miRNA: 3'- -UGG---CCuuCACCCC-----CGcUCCCU----UCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 16177 | 0.66 | 0.762552 |
Target: 5'- gGCgCGGgcGUGGcGGGgGuGGGcGGGGGc -3' miRNA: 3'- -UG-GCCuuCACC-CCCgCuCCCuUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 74451 | 0.66 | 0.751265 |
Target: 5'- gGCgGGggGUGGGGGCGccgcccccggcgcGGcgugccuGGgcGGGAu -3' miRNA: 3'- -UGgCCuuCACCCCCGC-------------UC-------CCuuCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 33124 | 0.66 | 0.743658 |
Target: 5'- uCCGcGAacAGaGGGGGagGGGGGAGGAGc -3' miRNA: 3'- uGGC-CU--UCaCCCCCg-CUCCCUUCUCu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 35255 | 0.66 | 0.740789 |
Target: 5'- gGCgGGAugggaggcagugguGGUgacGGGcGGUGAGGGAGGAcGAg -3' miRNA: 3'- -UGgCCU--------------UCA---CCC-CCGCUCCCUUCU-CU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 46025 | 0.66 | 0.734064 |
Target: 5'- uCCGGGAGcaggacGGGGGCGcGGu-AGAGGg -3' miRNA: 3'- uGGCCUUCa-----CCCCCGCuCCcuUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 22131 | 0.66 | 0.734064 |
Target: 5'- -gCGGuauacGGcGGGGG-GGGGGAAGGGGc -3' miRNA: 3'- ugGCCu----UCaCCCCCgCUCCCUUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 69318 | 0.66 | 0.7331 |
Target: 5'- -gCGGGAGcGGGGGUGAcGGcGAcagcgacGGAGAc -3' miRNA: 3'- ugGCCUUCaCCCCCGCU-CC-CU-------UCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 97962 | 0.66 | 0.724386 |
Target: 5'- --gGGAGGUGGcGGCGGagcggugcccGGGGAGAGu -3' miRNA: 3'- uggCCUUCACCcCCGCU----------CCCUUCUCu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 128060 | 0.66 | 0.724386 |
Target: 5'- aACCGGGccaUGGcGGGCGGGcGGgcGAGc -3' miRNA: 3'- -UGGCCUuc-ACC-CCCGCUC-CCuuCUCu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 34302 | 0.67 | 0.71463 |
Target: 5'- cGCCGGGAGcgagaccgacgGGGaGGcCGAGGGGccccaAGGGGc -3' miRNA: 3'- -UGGCCUUCa----------CCC-CC-GCUCCCU-----UCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 136392 | 0.67 | 0.71463 |
Target: 5'- uGCCGGGAcUGGGGGCGGacGuGGugucGGAGc -3' miRNA: 3'- -UGGCCUUcACCCCCGCU--C-CCu---UCUCu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 78420 | 0.67 | 0.71463 |
Target: 5'- -gUGGGcgauGGgGGGGGCGAuGGGGAGAa- -3' miRNA: 3'- ugGCCU----UCaCCCCCGCU-CCCUUCUcu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 15434 | 0.67 | 0.71463 |
Target: 5'- gGCCGGgcGcGGGccGGCGAuGGaGAAGAGc -3' miRNA: 3'- -UGGCCuuCaCCC--CCGCU-CC-CUUCUCu -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 48134 | 0.67 | 0.704807 |
Target: 5'- cGCgGGaAAGaGGGGG-GAGGGuggcGAGAGAg -3' miRNA: 3'- -UGgCC-UUCaCCCCCgCUCCC----UUCUCU- -5' |
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23835 | 5' | -59.3 | NC_005261.1 | + | 15026 | 0.67 | 0.694924 |
Target: 5'- gUCGGcGGGUgccGGGGGCGuAGcGGggGGGGu -3' miRNA: 3'- uGGCC-UUCA---CCCCCGC-UC-CCuuCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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