miRNA: 3'- -ACUgCGACCUUaGAGCCGGcuuCCGC- -5'
miRNA: 3'- aCUG-CGaCC----UUAGAGCCGG--------------CUUCCGC- -5'
Results 81 - 82 of 82 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23847 | 3' | -56.4 | NC_005261.1 | + | 2980 | 0.76 | 0.371037 |
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Target: 5'- cUGAgGCUGGGG-CUCGGCCu--GGCGg -3' miRNA: 3'- -ACUgCGACCUUaGAGCCGGcuuCCGC- -5' |
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| 23847 | 3' | -56.4 | NC_005261.1 | + | 2425 | 0.7 | 0.664026 |
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Target: 5'- cGGCgGCgGGccgcGAUCUCGGCCagcgccucggggucGAAGGCGa -3' miRNA: 3'- aCUG-CGaCC----UUAGAGCCGG--------------CUUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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