miRNA: 3'- -AGCa---GgCG------UAGAUGCUCUGC---ACGUAG- -5'
miRNA: 3'- agCAGGCGuaGa-UGCUCUGCaCGuaG- -5'
miRNA: 3'- aGCaGGCGuaGAUGCUCUGCaCGUag -5'
miRNA: 3'- aGCAGGCGUAGAU-GCUC----UGCa-CGUAG- -5'
miRNA: 3'- -AGCAGGCguAgAUGcucUGCACGUAG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23848 | 3' | -52.6 | NC_005261.1 | + | 120303 | 0.66 | 0.969894 |
|
Target: 5'- uUCGagggCgGCgaggcuGUCUGCGAGGCGcgcUGCGUCc -3' miRNA: 3'- -AGCa---GgCG------UAGAUGCUCUGC---ACGUAG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 121262 | 0.66 | 0.975498 |
|
Target: 5'- -gGUCCGCcgCagACGGGGCG-GCcgCc -3' miRNA: 3'- agCAGGCGuaGa-UGCUCUGCaCGuaG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 121423 | 0.71 | 0.837299 |
|
Target: 5'- cCG-CCGCcgCUACGGGGCG-GCGg- -3' miRNA: 3'- aGCaGGCGuaGAUGCUCUGCaCGUag -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 126789 | 0.75 | 0.620365 |
|
Target: 5'- gCGUCUGCGUCUGcCGAGagcaGCGcgGCGUCu -3' miRNA: 3'- aGCAGGCGUAGAU-GCUC----UGCa-CGUAG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 127551 | 0.67 | 0.947724 |
|
Target: 5'- aUUGUCCGguUgUAC---GCGUGCGUCg -3' miRNA: 3'- -AGCAGGCguAgAUGcucUGCACGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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