Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23854 | 3' | -47.5 | NC_005261.1 | + | 44220 | 0.67 | 0.99863 |
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Target: 5'- gCCCGGGAgcgCGCGCUgcUGGC-CGc--- -3' miRNA: 3'- -GGGUCUUaa-GCGUGA--ACCGaGUuauu -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 8711 | 0.67 | 0.998334 |
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Target: 5'- gCCAGcGUUCGCACaaaaCUCAAUAAg -3' miRNA: 3'- gGGUCuUAAGCGUGaaccGAGUUAUU- -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 8595 | 0.67 | 0.998334 |
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Target: 5'- gCCAGcGUUCGCACaaaaCUCAAUAAg -3' miRNA: 3'- gGGUCuUAAGCGUGaaccGAGUUAUU- -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 4737 | 0.67 | 0.998334 |
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Target: 5'- cCCCAGAGUcaUCGguCcccGGCUCGGg-- -3' miRNA: 3'- -GGGUCUUA--AGCguGaa-CCGAGUUauu -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 113244 | 0.67 | 0.997578 |
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Target: 5'- gCgCAGcaAGUUCGCGCgcgGGCUCAu--- -3' miRNA: 3'- -GgGUC--UUAAGCGUGaa-CCGAGUuauu -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 127148 | 0.72 | 0.955184 |
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Target: 5'- cCCCAGAGacgaCGCgACUcGGCUCGGUGu -3' miRNA: 3'- -GGGUCUUaa--GCG-UGAaCCGAGUUAUu -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 8899 | 0.74 | 0.892424 |
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Target: 5'- gCCAGuGUUCGCACaaaGCUCAAUAAu -3' miRNA: 3'- gGGUCuUAAGCGUGaacCGAGUUAUU- -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 24763 | 0.75 | 0.877449 |
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Target: 5'- gCCAGuGUUCGCACagaGCUCAAUAAg -3' miRNA: 3'- gGGUCuUAAGCGUGaacCGAGUUAUU- -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 25076 | 1.09 | 0.015841 |
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Target: 5'- uCCCAGAAUUCGCACUUGGCUCAAUAAu -3' miRNA: 3'- -GGGUCUUAAGCGUGAACCGAGUUAUU- -5' |
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| 23854 | 3' | -47.5 | NC_005261.1 | + | 24962 | 1.09 | 0.015841 |
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Target: 5'- uCCCAGAAUUCGCACUUGGCUCAAUAAu -3' miRNA: 3'- -GGGUCUUAAGCGUGAACCGAGUUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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