Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23858 | 3' | -46 | NC_005262.1 | + | 39123 | 0.66 | 0.997319 |
Target: 5'- gCUGUgauuuugucgcucaACGACGcUGGCGAcguAGC-UGUCa -3' miRNA: 3'- -GACA--------------UGCUGU-ACCGUUuu-UCGuACAG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 2073 | 0.66 | 0.996329 |
Target: 5'- -cGUGCGcACAUGGuCGcuuccGGGGGCAUGg- -3' miRNA: 3'- gaCAUGC-UGUACC-GU-----UUUUCGUACag -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 58796 | 0.67 | 0.992791 |
Target: 5'- gCUGggucgACGACGUgaucGGCGccguGAAGGCAUGg- -3' miRNA: 3'- -GACa----UGCUGUA----CCGU----UUUUCGUACag -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 61792 | 0.67 | 0.990207 |
Target: 5'- ---aGCGcCGUGGCGugGAGCAUG-Cg -3' miRNA: 3'- gacaUGCuGUACCGUuuUUCGUACaG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 34620 | 0.68 | 0.985033 |
Target: 5'- -gGUGCGACuacaacGGCAAGAAGCucgacccggcGUCg -3' miRNA: 3'- gaCAUGCUGua----CCGUUUUUCGua--------CAG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 60190 | 0.68 | 0.982911 |
Target: 5'- gUGgGCGGCAaccGGCAAGGAGCcgGcCg -3' miRNA: 3'- gACaUGCUGUa--CCGUUUUUCGuaCaG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 25338 | 0.68 | 0.98057 |
Target: 5'- -cGUAgGAUAcGGCGAcGAGCGUGcCg -3' miRNA: 3'- gaCAUgCUGUaCCGUUuUUCGUACaG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 27738 | 0.71 | 0.931599 |
Target: 5'- -aGUGCGGCAgguUGGCAGuaccGGGCGUacgGUCg -3' miRNA: 3'- gaCAUGCUGU---ACCGUUu---UUCGUA---CAG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 63026 | 0.71 | 0.919201 |
Target: 5'- -aGUACGGCAUcGGCAAcuGGCGcaaGUCc -3' miRNA: 3'- gaCAUGCUGUA-CCGUUuuUCGUa--CAG- -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 31943 | 0.75 | 0.799313 |
Target: 5'- gCUGUACGGCgguGUGGCGAcGAGCAa--- -3' miRNA: 3'- -GACAUGCUG---UACCGUUuUUCGUacag -5' |
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23858 | 3' | -46 | NC_005262.1 | + | 63628 | 1.12 | 0.006051 |
Target: 5'- gCUGUACGACAUGGCAAAAAGCAUGUCg -3' miRNA: 3'- -GACAUGCUGUACCGUUUUUCGUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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