Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2386 | 3' | -39.8 | NC_001416.1 | + | 42195 | 0.66 | 0.999999 |
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Target: 5'- --cGGUGGCuAAGAcgUcGUGCGAGGa -3' miRNA: 3'- aaaCUACUGuUUUUuaAuCGCGUUCU- -5' |
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| 2386 | 3' | -39.8 | NC_001416.1 | + | 19952 | 0.66 | 0.999998 |
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Target: 5'- -cUGAUGGUGAAGAggUGGCGCGu-- -3' miRNA: 3'- aaACUACUGUUUUUuaAUCGCGUucu -5' |
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| 2386 | 3' | -39.8 | NC_001416.1 | + | 10492 | 0.68 | 0.999982 |
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Target: 5'- -cUGAUGACAguaucAGAAGggAuCGCAGGAg -3' miRNA: 3'- aaACUACUGU-----UUUUUaaUcGCGUUCU- -5' |
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| 2386 | 3' | -39.8 | NC_001416.1 | + | 27615 | 0.72 | 0.997145 |
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Target: 5'- -gUGAccUGuaACAGAGcAUUAGCGCAAGGu -3' miRNA: 3'- aaACU--AC--UGUUUUuUAAUCGCGUUCU- -5' |
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| 2386 | 3' | -39.8 | NC_001416.1 | + | 32933 | 0.74 | 0.989911 |
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Target: 5'- gUUUGAUGACGAuguuGAGUUucaggAGCGCAuggcAGAa -3' miRNA: 3'- -AAACUACUGUUu---UUUAA-----UCGCGU----UCU- -5' |
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| 2386 | 3' | -39.8 | NC_001416.1 | + | 27501 | 1.1 | 0.032916 |
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Target: 5'- gUUUGAUGACAAAAAAUUAGCGCAAGAa -3' miRNA: 3'- -AAACUACUGUUUUUUAAUCGCGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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