Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23863 | 3' | -59.7 | NC_005262.1 | + | 46892 | 0.66 | 0.503181 |
Target: 5'- uGCGguGCGGUGCgGgcggugcGGUGUGGuGCGg -3' miRNA: 3'- -UGCuuCGUCGCGgCa------CCGCACCuCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 15442 | 0.66 | 0.493134 |
Target: 5'- cCGGccgccAGCAGCGCCGggucGGuCGUGccGAGCu -3' miRNA: 3'- uGCU-----UCGUCGCGGCa---CC-GCAC--CUCGu -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 56508 | 0.66 | 0.492134 |
Target: 5'- aGCGAggAGCAucuggacGCGCCGUGGUucccggcauccGUcGAGCAg -3' miRNA: 3'- -UGCU--UCGU-------CGCGGCACCG-----------CAcCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 59601 | 0.66 | 0.48318 |
Target: 5'- uACGAu-CAG-GCCGUGGuCGUGG-GCGa -3' miRNA: 3'- -UGCUucGUCgCGGCACC-GCACCuCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 52720 | 0.67 | 0.454881 |
Target: 5'- gACGAGGCcGCGCUGcugacgaaguucacgGGC-UGGGGCGc -3' miRNA: 3'- -UGCUUCGuCGCGGCa--------------CCGcACCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 59219 | 0.67 | 0.434958 |
Target: 5'- cACGcccGAGCAGCGCCGccgcUGGCGcGaGAuGCGc -3' miRNA: 3'- -UGC---UUCGUCGCGGC----ACCGCaC-CU-CGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 10584 | 0.68 | 0.398455 |
Target: 5'- cGCGGAGCAGCGC---GGCGc-GAGCGg -3' miRNA: 3'- -UGCUUCGUCGCGgcaCCGCacCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 4573 | 0.68 | 0.38964 |
Target: 5'- cGCGccagcGGCGGCGCUGcucgGGCGU-GAGCGc -3' miRNA: 3'- -UGCu----UCGUCGCGGCa---CCGCAcCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 52155 | 0.68 | 0.372398 |
Target: 5'- cGCGAcGcCGGCGCCGccgagccgaUGGUGcagGGGGCAg -3' miRNA: 3'- -UGCUuC-GUCGCGGC---------ACCGCa--CCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 62377 | 0.68 | 0.372398 |
Target: 5'- gACGAAGCGGCcgcgGUCGUGGCGgaucaucAGCGc -3' miRNA: 3'- -UGCUUCGUCG----CGGCACCGCacc----UCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 52616 | 0.68 | 0.363974 |
Target: 5'- cACGAucacGGCGGCGUCGUcGGCGuUGucGCAg -3' miRNA: 3'- -UGCU----UCGUCGCGGCA-CCGC-ACcuCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 14037 | 0.69 | 0.355683 |
Target: 5'- cCGGAGCcucgggcgcgGGCGCCGccGGCGUcGGuGCGg -3' miRNA: 3'- uGCUUCG----------UCGCGGCa-CCGCA-CCuCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 17554 | 0.69 | 0.339503 |
Target: 5'- cGCGAGGCGGCaGCCGcGGCGgcgcGCGa -3' miRNA: 3'- -UGCUUCGUCG-CGGCaCCGCaccuCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 17598 | 0.69 | 0.339503 |
Target: 5'- gGCGgcGCAGCGCgCG-GaGC-UGGAGCGc -3' miRNA: 3'- -UGCuuCGUCGCG-GCaC-CGcACCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 14519 | 0.69 | 0.338708 |
Target: 5'- gGCGAucggcaacagcauGGCAGUGCCGUGcauGCGcUGGAucgGCAg -3' miRNA: 3'- -UGCU-------------UCGUCGCGGCAC---CGC-ACCU---CGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 42768 | 0.69 | 0.329276 |
Target: 5'- uCGAcGGCAGCGCUGUGGUGcgaaaaucguaucaUGGuucGGCAa -3' miRNA: 3'- uGCU-UCGUCGCGGCACCGC--------------ACC---UCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 4094 | 0.69 | 0.323864 |
Target: 5'- gGCGAcGUAGCGCCc--GCGUGGcGGCAc -3' miRNA: 3'- -UGCUuCGUCGCGGcacCGCACC-UCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 17220 | 0.7 | 0.308027 |
Target: 5'- gACGgcGCuGGCGUCGUGGuCGUcgugggcgucgcgGGAGCGg -3' miRNA: 3'- -UGCuuCG-UCGCGGCACC-GCA-------------CCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 10486 | 0.71 | 0.267384 |
Target: 5'- gGCGAccagcAGCAGCGCCagcaUGGCGgccagcagcagcgcgGGGGCGg -3' miRNA: 3'- -UGCU-----UCGUCGCGGc---ACCGCa--------------CCUCGU- -5' |
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23863 | 3' | -59.7 | NC_005262.1 | + | 52393 | 0.75 | 0.125099 |
Target: 5'- cCGgcGCGGCGCgGcuucGGCGUGGAGCc -3' miRNA: 3'- uGCuuCGUCGCGgCa---CCGCACCUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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