Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23868 | 3' | -52.8 | NC_005262.1 | + | 49560 | 0.66 | 0.874284 |
Target: 5'- --cUGCGuCCUGCUCGUgCGUGGUCg- -3' miRNA: 3'- cacAUGUcGGACGAGCA-GUACUAGgc -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 4582 | 0.66 | 0.849455 |
Target: 5'- ---gGCGGCgCUGCUCGggCGUGAgcgcCCGc -3' miRNA: 3'- cacaUGUCG-GACGAGCa-GUACUa---GGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 53131 | 0.66 | 0.849455 |
Target: 5'- -cGUuCGGCCUGCUCGUUugcGAUgUCGa -3' miRNA: 3'- caCAuGUCGGACGAGCAGua-CUA-GGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 6415 | 0.66 | 0.8407 |
Target: 5'- -gGUACAGCUUGCUgaucgUGUCGgcGUCCu -3' miRNA: 3'- caCAUGUCGGACGA-----GCAGUacUAGGc -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 13254 | 0.66 | 0.8407 |
Target: 5'- -gGUGCgcgAGCCUGCUCaUCAgcaccaGUCCGa -3' miRNA: 3'- caCAUG---UCGGACGAGcAGUac----UAGGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 47790 | 0.68 | 0.773681 |
Target: 5'- ------cGCCUGCUCGUCGUcGGUgCCGc -3' miRNA: 3'- cacauguCGGACGAGCAGUA-CUA-GGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 8408 | 0.68 | 0.751966 |
Target: 5'- --aUGCGGCgcucgauCUGCUUGcgCGUGAUCCGg -3' miRNA: 3'- cacAUGUCG-------GACGAGCa-GUACUAGGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 53886 | 0.7 | 0.643987 |
Target: 5'- gGUGUG-AGCUcgaucgugUGCUCGcCAUGAUCCa -3' miRNA: 3'- -CACAUgUCGG--------ACGAGCaGUACUAGGc -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 25744 | 0.71 | 0.566281 |
Target: 5'- ---aGCGGCCgugccGUUCGUCGUGAUgCCGa -3' miRNA: 3'- cacaUGUCGGa----CGAGCAGUACUA-GGC- -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 43544 | 0.72 | 0.533621 |
Target: 5'- aUGUGCcGCCUGC-CGUCcgGAUUCc -3' miRNA: 3'- cACAUGuCGGACGaGCAGuaCUAGGc -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 34579 | 0.73 | 0.45038 |
Target: 5'- cGUGUGCGcGCCgUGCggCGcCGUGAUCCa -3' miRNA: 3'- -CACAUGU-CGG-ACGa-GCaGUACUAGGc -5' |
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23868 | 3' | -52.8 | NC_005262.1 | + | 60189 | 1.1 | 0.001622 |
Target: 5'- cGUGUACAGCCUGCUCGUCAUGAUCCGg -3' miRNA: 3'- -CACAUGUCGGACGAGCAGUACUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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