Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2387 | 5' | -57.6 | NC_001416.1 | + | 10677 | 0.66 | 0.50421 |
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Target: 5'- -cGUACCAgaaaaaugACGCCUGACU-CUggCCAUCu -3' miRNA: 3'- uuCGUGGU--------UGCGGACUGAcGG--GGUAG- -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 13871 | 0.69 | 0.300851 |
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Target: 5'- -uGCGCCAGCGgCUGAucaccucCUGCUCCGgaUCg -3' miRNA: 3'- uuCGUGGUUGCgGACU-------GACGGGGU--AG- -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 9288 | 0.71 | 0.23884 |
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Target: 5'- --aCGCCAgcaGCGCCUG-CUGCCCCugcUCu -3' miRNA: 3'- uucGUGGU---UGCGGACuGACGGGGu--AG- -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 13533 | 0.73 | 0.187368 |
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Target: 5'- aGAGCACC-ACGCUggccGACUGCUCCGc- -3' miRNA: 3'- -UUCGUGGuUGCGGa---CUGACGGGGUag -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 37752 | 1.06 | 0.000598 |
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Target: 5'- aAAGCACCAACGCCUGACUGCCCCAUCc -3' miRNA: 3'- -UUCGUGGUUGCGGACUGACGGGGUAG- -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 39879 | 0.7 | 0.265285 |
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Target: 5'- gAAGCAuCCGuuacCGCCgGACUGCCaaaCGUCa -3' miRNA: 3'- -UUCGU-GGUu---GCGGaCUGACGGg--GUAG- -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 4550 | 0.67 | 0.42298 |
|
Target: 5'- cGGUGuCCGGCgacaGCCUGACUGCCCa--- -3' miRNA: 3'- uUCGU-GGUUG----CGGACUGACGGGguag -5' |
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| 2387 | 5' | -57.6 | NC_001416.1 | + | 7014 | 0.67 | 0.42298 |
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Target: 5'- aAGGCAUUAACGCCU--CUGCCCguUa -3' miRNA: 3'- -UUCGUGGUUGCGGAcuGACGGGguAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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