Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23871 | 5' | -57.1 | NC_005262.1 | + | 52638 | 0.66 | 0.713917 |
Target: 5'- gCGuuGUCgca-GGGCCGCCCgugcgaccGGAGCCa -3' miRNA: 3'- -GCu-CAGauagCUUGGCGGG--------CCUCGGc -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 23144 | 0.66 | 0.703528 |
Target: 5'- uGGGUCggGUCGcugacGCCguGCCCGuGGCCGg -3' miRNA: 3'- gCUCAGa-UAGCu----UGG--CGGGCcUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 48539 | 0.66 | 0.703528 |
Target: 5'- uCGcGUC--UCGAugCGCa-GGAGCCGg -3' miRNA: 3'- -GCuCAGauAGCUugGCGggCCUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 45384 | 0.66 | 0.703528 |
Target: 5'- -aAGUCgcgcucAUUGAGgCGCUCGGaAGCCGg -3' miRNA: 3'- gcUCAGa-----UAGCUUgGCGGGCC-UCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 6639 | 0.66 | 0.67201 |
Target: 5'- gGGGUCgccggggaUGUCGAccgacacgGCCGCgauguacucgCCGGAGCCc -3' miRNA: 3'- gCUCAG--------AUAGCU--------UGGCG----------GGCCUCGGc -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 38543 | 0.67 | 0.650806 |
Target: 5'- -cGGUCgcggugacgAUCGGcACCGCCgCGG-GCCGg -3' miRNA: 3'- gcUCAGa--------UAGCU-UGGCGG-GCCuCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 61121 | 0.67 | 0.639111 |
Target: 5'- uGAGcuugCUAcggCGGccaggacGCCGCCCGGcGCCGc -3' miRNA: 3'- gCUCa---GAUa--GCU-------UGGCGGGCCuCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 23435 | 0.67 | 0.629537 |
Target: 5'- uCGAcGUCgccgacgAUCGAcaucagcgcGCCGgCCGuGAGCCGa -3' miRNA: 3'- -GCU-CAGa------UAGCU---------UGGCgGGC-CUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 1045 | 0.67 | 0.629537 |
Target: 5'- uCGAcaaUAggcgCGAuCgGCCCGGAGCCGa -3' miRNA: 3'- -GCUcagAUa---GCUuGgCGGGCCUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 44663 | 0.67 | 0.597672 |
Target: 5'- cCGGGUucCUAUCGAcauGCUGCCCGaccgcgacaAGCCGu -3' miRNA: 3'- -GCUCA--GAUAGCU---UGGCGGGCc--------UCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 22448 | 0.68 | 0.576551 |
Target: 5'- --cGUCcg-CGAGCUGCcauCCGGGGCCGu -3' miRNA: 3'- gcuCAGauaGCUUGGCG---GGCCUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 9713 | 0.68 | 0.576551 |
Target: 5'- gCGGGUCguucUUGAACCGCUucgucacggucaCGGuGCCGu -3' miRNA: 3'- -GCUCAGau--AGCUUGGCGG------------GCCuCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 37562 | 0.69 | 0.534878 |
Target: 5'- aCGAGgc---CGAGCC-CCUGGAGCCa -3' miRNA: 3'- -GCUCagauaGCUUGGcGGGCCUCGGc -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 59128 | 0.69 | 0.524619 |
Target: 5'- cCGAGg----CGAACCGgCCGG-GCCGc -3' miRNA: 3'- -GCUCagauaGCUUGGCgGGCCuCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 12264 | 0.69 | 0.524619 |
Target: 5'- uCGGGcacCUGUCGAucGCgGCCgGcGAGCCGa -3' miRNA: 3'- -GCUCa--GAUAGCU--UGgCGGgC-CUCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 4331 | 0.69 | 0.514435 |
Target: 5'- gCGAGcCUcgCGGGCCGUCCaucGGCCGu -3' miRNA: 3'- -GCUCaGAuaGCUUGGCGGGcc-UCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 28716 | 0.69 | 0.504332 |
Target: 5'- aCGAGUUccgccgCGAggccGCgCGCCCGGAcGCCGu -3' miRNA: 3'- -GCUCAGaua---GCU----UG-GCGGGCCU-CGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 34454 | 0.7 | 0.427014 |
Target: 5'- ---uUCgcucCGGGCUGCCCGGAGCCu -3' miRNA: 3'- gcucAGaua-GCUUGGCGGGCCUCGGc -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 49836 | 0.71 | 0.391077 |
Target: 5'- gGAG-CgcgCGAAUgGCCCGGuGCCGg -3' miRNA: 3'- gCUCaGauaGCUUGgCGGGCCuCGGC- -5' |
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23871 | 5' | -57.1 | NC_005262.1 | + | 2532 | 0.71 | 0.382406 |
Target: 5'- uCGAGUCUGUcaCGAACUgGCCCaGGccGCCGg -3' miRNA: 3'- -GCUCAGAUA--GCUUGG-CGGG-CCu-CGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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