Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23872 | 3' | -55.9 | NC_005262.1 | + | 4406 | 0.66 | 0.688807 |
Target: 5'- gAugUCGCGCgccugcUGCGCgAGCGcCUGGAg -3' miRNA: 3'- -UugGGCGUGa-----ACGCG-UUGCuGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 32052 | 0.66 | 0.688807 |
Target: 5'- gGACCgUGCGCUUGCGCGacauGCGACcgccGGc- -3' miRNA: 3'- -UUGG-GCGUGAACGCGU----UGCUGa---CCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 18680 | 0.66 | 0.677819 |
Target: 5'- -uCuuGCcCUUGCGCAuauCGGCUGGc- -3' miRNA: 3'- uuGggCGuGAACGCGUu--GCUGACCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 20351 | 0.66 | 0.655722 |
Target: 5'- cGAUCCGCACaaGCGCGucgccguCGAUcGGAUa -3' miRNA: 3'- -UUGGGCGUGaaCGCGUu------GCUGaCCUA- -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 63643 | 0.66 | 0.633536 |
Target: 5'- uGACCCaGCGgUUGCggGCGAacUGGCUGGAa -3' miRNA: 3'- -UUGGG-CGUgAACG--CGUU--GCUGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 46822 | 0.67 | 0.623546 |
Target: 5'- aGGCCCGCGCgcucgccgagaaaaGCGCGccgaGCGuGCUGGAc -3' miRNA: 3'- -UUGGGCGUGaa------------CGCGU----UGC-UGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 14348 | 0.67 | 0.622436 |
Target: 5'- uGCgaCGUGCUUGCGCGcguCGACUGGc- -3' miRNA: 3'- uUGg-GCGUGAACGCGUu--GCUGACCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 23454 | 0.67 | 0.600271 |
Target: 5'- cGACUgGCGCaUGCGCGGCcuggacgaauGGCUGGGc -3' miRNA: 3'- -UUGGgCGUGaACGCGUUG----------CUGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 6102 | 0.67 | 0.589226 |
Target: 5'- uGAUCCGCACgUUGCGgAACGGC-GGc- -3' miRNA: 3'- -UUGGGCGUG-AACGCgUUGCUGaCCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 29219 | 0.68 | 0.556344 |
Target: 5'- cGGCgCCGCACggcGCGCAcACGACguucGGAUc -3' miRNA: 3'- -UUG-GGCGUGaa-CGCGU-UGCUGa---CCUA- -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 28801 | 0.68 | 0.556344 |
Target: 5'- gGGCCCGCGCUgcUGCcCGGCuGcCUGGAg -3' miRNA: 3'- -UUGGGCGUGA--ACGcGUUG-CuGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 26994 | 0.69 | 0.492426 |
Target: 5'- cGCCCGC-CgaGCGCGcCGcACUGGAg -3' miRNA: 3'- uUGGGCGuGaaCGCGUuGC-UGACCUa -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 48494 | 0.69 | 0.461744 |
Target: 5'- cGCCCGCcCaUGCGCGgcgaaucgACGACUGGc- -3' miRNA: 3'- uUGGGCGuGaACGCGU--------UGCUGACCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 38728 | 0.69 | 0.451741 |
Target: 5'- uACCCGaCGCUcaGCGCuGGCGACUGGu- -3' miRNA: 3'- uUGGGC-GUGAa-CGCG-UUGCUGACCua -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 56330 | 0.71 | 0.350299 |
Target: 5'- uAGCCCGCug--GCGUggcaaGACGGCUGGGUa -3' miRNA: 3'- -UUGGGCGugaaCGCG-----UUGCUGACCUA- -5' |
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23872 | 3' | -55.9 | NC_005262.1 | + | 59090 | 1.04 | 0.001829 |
Target: 5'- cAACCCGCACUUGCGCAACGACUGGAUg -3' miRNA: 3'- -UUGGGCGUGAACGCGUUGCUGACCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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