Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23872 | 5' | -58.5 | NC_005262.1 | + | 49938 | 0.66 | 0.594949 |
Target: 5'- aCGA-CCGGCC-CGG-AUGGCgugacGUUCGa -3' miRNA: 3'- -GCUaGGCCGGcGCCgUACCGa----CAAGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 52036 | 0.66 | 0.584328 |
Target: 5'- aGGUCgGGCCGCGcCA--GUUGUUCGa -3' miRNA: 3'- gCUAGgCCGGCGCcGUacCGACAAGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 32518 | 0.66 | 0.573744 |
Target: 5'- gCGAUCuUGGCCGCGGaCGacugcGGCUGccgaUCGa -3' miRNA: 3'- -GCUAG-GCCGGCGCC-GUa----CCGACa---AGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 39214 | 0.67 | 0.542293 |
Target: 5'- uCGAUCCGGCCagcaagcagGCGGCcgGGaauuaUGcgaagUCGa -3' miRNA: 3'- -GCUAGGCCGG---------CGCCGuaCCg----ACa----AGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 15769 | 0.67 | 0.531932 |
Target: 5'- gCGG-CCGGCUGCuGCGUGGuCUGg--- -3' miRNA: 3'- -GCUaGGCCGGCGcCGUACC-GACaagu -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 41280 | 0.67 | 0.521643 |
Target: 5'- aGAUUCGGCCGCG-CGUGgGCgacaUCAa -3' miRNA: 3'- gCUAGGCCGGCGCcGUAC-CGaca-AGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 60384 | 0.67 | 0.511433 |
Target: 5'- aGGUcgCCGGCCgcaagaucgucgGCGGCAUGGCgcacGUggUCGa -3' miRNA: 3'- gCUA--GGCCGG------------CGCCGUACCGa---CA--AGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 40457 | 0.67 | 0.501306 |
Target: 5'- cCGAcaucCCGGCCGUgcaugGGCcgGGCUGg--- -3' miRNA: 3'- -GCUa---GGCCGGCG-----CCGuaCCGACaagu -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 9301 | 0.67 | 0.501306 |
Target: 5'- uCGGUCgGGCCcaGCGGCuucGGCUGc--- -3' miRNA: 3'- -GCUAGgCCGG--CGCCGua-CCGACaagu -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 57685 | 0.67 | 0.491269 |
Target: 5'- gCGcgCCGGgCGCGGCAaGGUgcuUUCGg -3' miRNA: 3'- -GCuaGGCCgGCGCCGUaCCGac-AAGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 20445 | 0.68 | 0.481326 |
Target: 5'- aCGGuUCCGGCaccguCGCcGCGUGGgCUGUUCc -3' miRNA: 3'- -GCU-AGGCCG-----GCGcCGUACC-GACAAGu -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 46922 | 0.69 | 0.405662 |
Target: 5'- gCGGcCCGGCgCGCGGCcgGGCgccugCAu -3' miRNA: 3'- -GCUaGGCCG-GCGCCGuaCCGacaa-GU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 20113 | 0.7 | 0.362336 |
Target: 5'- gCGaAUUCGGCC-CGGCuUGGCUugcGUUCAg -3' miRNA: 3'- -GC-UAGGCCGGcGCCGuACCGA---CAAGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 58811 | 0.7 | 0.345928 |
Target: 5'- uGAUCgGcGCCGUgaaGGCAUGGCUGcugCGg -3' miRNA: 3'- gCUAGgC-CGGCG---CCGUACCGACaa-GU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 4689 | 0.71 | 0.307278 |
Target: 5'- gGAUCgGGCCGCGGCAUccagucGUUGcgCAa -3' miRNA: 3'- gCUAGgCCGGCGCCGUAc-----CGACaaGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 37022 | 0.73 | 0.22821 |
Target: 5'- ---aUCGGCgGCGGUucAUGGCUGUUCu -3' miRNA: 3'- gcuaGGCCGgCGCCG--UACCGACAAGu -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 62378 | 0.76 | 0.146203 |
Target: 5'- aCGAagCGGCCGCGGuCGUGGCgGaUCAu -3' miRNA: 3'- -GCUagGCCGGCGCC-GUACCGaCaAGU- -5' |
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23872 | 5' | -58.5 | NC_005262.1 | + | 59125 | 1.09 | 0.000614 |
Target: 5'- cCGAUCCGGCCGCGGCAUGGCUGUUCAa -3' miRNA: 3'- -GCUAGGCCGGCGCCGUACCGACAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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