Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23874 | 5' | -53.4 | NC_005262.1 | + | 36151 | 0.66 | 0.805441 |
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Target: 5'- ---aGcGCGAUCGAcCAGCUCGAGCAg -3' miRNA: 3'- uagaCcUGCUAGUU-GUCGGGUUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 9499 | 0.66 | 0.805441 |
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Target: 5'- -aCUGGuGCGAUUuGCGGCCCGAccGCu- -3' miRNA: 3'- uaGACC-UGCUAGuUGUCGGGUU--CGua -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 59685 | 0.66 | 0.785436 |
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Target: 5'- uUgUGGACGAUCGGCGuGCCgccacgCGGGCGc -3' miRNA: 3'- uAgACCUGCUAGUUGU-CGG------GUUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 4431 | 0.66 | 0.775173 |
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Target: 5'- uUCUGGGCu-UCAcgggGCGGCuCCGGGCGg -3' miRNA: 3'- uAGACCUGcuAGU----UGUCG-GGUUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 49640 | 0.66 | 0.764754 |
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Target: 5'- --gUGGccucGCGAUCGGCGGCCUgcaAGGCGg -3' miRNA: 3'- uagACC----UGCUAGUUGUCGGG---UUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 3738 | 0.67 | 0.743494 |
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Target: 5'- -cCUGGAUGGguUCcgcGCGGCUCAGGCGa -3' miRNA: 3'- uaGACCUGCU--AGu--UGUCGGGUUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 56340 | 0.68 | 0.643343 |
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Target: 5'- cGUCUGGuCGAgggCGGCGuGCCCcgcGAGCAg -3' miRNA: 3'- -UAGACCuGCUa--GUUGU-CGGG---UUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 45392 | 0.7 | 0.575523 |
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Target: 5'- cAUCUGGGCGGcgCAGCAGCa--GGCAg -3' miRNA: 3'- -UAGACCUGCUa-GUUGUCGgguUCGUa -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 27954 | 0.71 | 0.509441 |
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Target: 5'- cAUCUGGGCGGUCG-CGGCgCCGAcGCc- -3' miRNA: 3'- -UAGACCUGCUAGUuGUCG-GGUU-CGua -5' |
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| 23874 | 5' | -53.4 | NC_005262.1 | + | 58806 | 1.03 | 0.00323 |
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Target: 5'- gAUCUGGACGAUCAACAGCCCAAGCAUc -3' miRNA: 3'- -UAGACCUGCUAGUUGUCGGGUUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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