Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23877 | 5' | -55 | NC_005262.1 | + | 4561 | 0.66 | 0.758551 |
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Target: 5'- gAGCGUGCAGcagcuCCUCGAgCUGUUc -3' miRNA: 3'- -UCGCACGUCuacuuGGGGCUaGACGA- -5' |
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| 23877 | 5' | -55 | NC_005262.1 | + | 10575 | 0.68 | 0.651104 |
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Target: 5'- uAGuCGUGCAGcAUGAACCCCuGUUucuUGUa -3' miRNA: 3'- -UC-GCACGUC-UACUUGGGGcUAG---ACGa -5' |
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| 23877 | 5' | -55 | NC_005262.1 | + | 12875 | 0.7 | 0.482959 |
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Target: 5'- uGcCGUGCAcGGUGAcgcucgugcgcaucaGCCCCGGcCUGCUg -3' miRNA: 3'- uC-GCACGU-CUACU---------------UGGGGCUaGACGA- -5' |
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| 23877 | 5' | -55 | NC_005262.1 | + | 53095 | 0.73 | 0.365445 |
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Target: 5'- cGCGUGCGcgc-GGCCUCGAUCUGCg -3' miRNA: 3'- uCGCACGUcuacUUGGGGCUAGACGa -5' |
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| 23877 | 5' | -55 | NC_005262.1 | + | 37714 | 0.74 | 0.323968 |
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Target: 5'- cGGCGUGCAGAUGGGgCgUGAagggCUGCg -3' miRNA: 3'- -UCGCACGUCUACUUgGgGCUa---GACGa -5' |
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| 23877 | 5' | -55 | NC_005262.1 | + | 57952 | 1.07 | 0.001526 |
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Target: 5'- cAGCGUGCAGAUGAACCCCGAUCUGCUc -3' miRNA: 3'- -UCGCACGUCUACUUGGGGCUAGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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