Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2388 | 5' | -44.6 | NC_001416.1 | + | 39028 | 1.17 | 0.004304 |
Target: 5'- aGGGAUAAAACAUCCCUCAAAUUGGGGg -3' miRNA: 3'- -CCCUAUUUUGUAGGGAGUUUAACCCC- -5' |
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2388 | 5' | -44.6 | NC_001416.1 | + | 39053 | 0.74 | 0.864792 |
Target: 5'- nGGAUu--GCuAUCCCUCAAAacagGGGGa -3' miRNA: 3'- cCCUAuuuUG-UAGGGAGUUUaa--CCCC- -5' |
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2388 | 5' | -44.6 | NC_001416.1 | + | 45109 | 0.69 | 0.990301 |
Target: 5'- --aAUAGAGCAaaUCCC-CuuAUUGGGGg -3' miRNA: 3'- cccUAUUUUGU--AGGGaGuuUAACCCC- -5' |
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2388 | 5' | -44.6 | NC_001416.1 | + | 38989 | 0.67 | 0.99701 |
Target: 5'- uGGu----GCAUCCCUCAAAacGaGGGa -3' miRNA: 3'- cCCuauuuUGUAGGGAGUUUaaC-CCC- -5' |
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2388 | 5' | -44.6 | NC_001416.1 | + | 10843 | 0.66 | 0.999138 |
Target: 5'- cGGcgGGAugAUCCC-CAuGUUcaGGGGg -3' miRNA: 3'- cCCuaUUUugUAGGGaGUuUAA--CCCC- -5' |
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2388 | 5' | -44.6 | NC_001416.1 | + | 39092 | 0.8 | 0.613874 |
Target: 5'- aGGGAUGuuuUAUCCCUCGuuUUaGGGGa -3' miRNA: 3'- -CCCUAUuuuGUAGGGAGUuuAA-CCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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