Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23880 | 3' | -51.9 | NC_005262.1 | + | 45999 | 0.66 | 0.915888 |
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Target: 5'- cGUGUUcgCGuuCCUGcacgcgcaaGGCGUCGCGCu -3' miRNA: 3'- cCACAAa-GC--GGACaaa------CCGCAGCGUG- -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 19872 | 0.67 | 0.864943 |
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Target: 5'- cGUGUUguagUCgGCCgcggUUGGCGcCGCGCu -3' miRNA: 3'- cCACAA----AG-CGGaca-AACCGCaGCGUG- -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 45872 | 0.67 | 0.864943 |
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Target: 5'- -----cUCGCCg--UUGGCGUCgGCGCc -3' miRNA: 3'- ccacaaAGCGGacaAACCGCAG-CGUG- -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 6556 | 0.69 | 0.761673 |
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Target: 5'- aGGUugUUCaCCUGaUUGGCGgCGCGCg -3' miRNA: 3'- -CCAcaAAGcGGACaAACCGCaGCGUG- -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 9797 | 0.7 | 0.730013 |
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Target: 5'- cGUGcUUCGCC-GUgaaGGCGUCGUAg -3' miRNA: 3'- cCACaAAGCGGaCAaa-CCGCAGCGUg -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 39411 | 0.73 | 0.564288 |
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Target: 5'- cGUGcg-CGCCgccgGUUUGGCGUCGaCAg -3' miRNA: 3'- cCACaaaGCGGa---CAAACCGCAGC-GUg -5' |
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| 23880 | 3' | -51.9 | NC_005262.1 | + | 56651 | 1.11 | 0.001868 |
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Target: 5'- gGGUGUUUCGCCUGUUUGGCGUCGCACc -3' miRNA: 3'- -CCACAAAGCGGACAAACCGCAGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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