Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23884 | 5' | -62.2 | NC_005262.1 | + | 28536 | 0.66 | 0.419475 |
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Target: 5'- uGGUCgUGcCGUucGCCGGGGGcGCCc -3' miRNA: 3'- gUCAGgACuGCGcuCGGCCUCC-CGGa -5' |
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| 23884 | 5' | -62.2 | NC_005262.1 | + | 19948 | 0.67 | 0.335973 |
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Target: 5'- aGGUCCUGccggccaccgccGCGCGcGGCCGGcccGcGGCCg -3' miRNA: 3'- gUCAGGAC------------UGCGC-UCGGCCu--C-CCGGa -5' |
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| 23884 | 5' | -62.2 | NC_005262.1 | + | 4672 | 0.68 | 0.278381 |
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Target: 5'- aCAG-CCaUGcCGCG-GCCGGAucGGGCCg -3' miRNA: 3'- -GUCaGG-ACuGCGCuCGGCCU--CCCGGa -5' |
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| 23884 | 5' | -62.2 | NC_005262.1 | + | 50828 | 0.68 | 0.278381 |
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Target: 5'- gCGGUCgUcGACGcCGAGCCGGucGGCg- -3' miRNA: 3'- -GUCAGgA-CUGC-GCUCGGCCucCCGga -5' |
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| 23884 | 5' | -62.2 | NC_005262.1 | + | 17355 | 0.72 | 0.168838 |
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Target: 5'- gGGUCC-GGCGCGGGCggcgCGGcGGGCCc -3' miRNA: 3'- gUCAGGaCUGCGCUCG----GCCuCCCGGa -5' |
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| 23884 | 5' | -62.2 | NC_005262.1 | + | 55771 | 1.06 | 0.00045 |
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Target: 5'- uCAGUCCUGACGCGAGCCGGAGGGCCUu -3' miRNA: 3'- -GUCAGGACUGCGCUCGGCCUCCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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