miRNA: 3'- uCACCACGCCuuaGCCGUGguaUGGC- -5'
miRNA: 3'- ucaCCACG-CCUUAGCCGUGGua-UGGC- -5'
miRNA: 3'- uCACCACGCCuUAGC----CGUGGUaUGGc -5'
miRNA: 3'- -UCACCACGCCuuaGCCGUGgUAUGGC- -5'
miRNA: 3'- -UCACCACGCCUUAGCCGUGGUAUGGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23888 | 3' | -55 | NC_005262.1 | + | 46916 | 0.74 | 0.323932 |
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Target: 5'- uGUGGUGCGGcc-CGGCGCgcgGCCGg -3' miRNA: 3'- uCACCACGCCuuaGCCGUGguaUGGC- -5' |
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| 23888 | 3' | -55 | NC_005262.1 | + | 21860 | 0.76 | 0.252477 |
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Target: 5'- --aGGUGCaGGGAUCGGCGCUgaaGCCGa -3' miRNA: 3'- ucaCCACG-CCUUAGCCGUGGua-UGGC- -5' |
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| 23888 | 3' | -55 | NC_005262.1 | + | 57750 | 0.77 | 0.210562 |
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Target: 5'- cGUGGUGCGGuAUCGugccGCACCAcACCa -3' miRNA: 3'- uCACCACGCCuUAGC----CGUGGUaUGGc -5' |
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| 23888 | 3' | -55 | NC_005262.1 | + | 6038 | 0.84 | 0.080319 |
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Target: 5'- uGGUGGUGUGGug-CGGCACgAUACCGc -3' miRNA: 3'- -UCACCACGCCuuaGCCGUGgUAUGGC- -5' |
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| 23888 | 3' | -55 | NC_005262.1 | + | 54318 | 1.08 | 0.001438 |
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Target: 5'- cAGUGGUGCGGAAUCGGCACCAUACCGu -3' miRNA: 3'- -UCACCACGCCUUAGCCGUGGUAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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