Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 239 | 3' | -52.3 | AC_000008.1 | + | 6625 | 0.67 | 0.733636 |
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Target: 5'- gCCUGCUGCccGggGUCUUuaCCGUggaCGCa -3' miRNA: 3'- -GGACGAUGa-CaaCAGAA--GGCAg--GCGg -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 15155 | 0.68 | 0.67757 |
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Target: 5'- gCCgcgGCUGCcgcGUUGgaguucUCUUCCGcgCCGCUg -3' miRNA: 3'- -GGa--CGAUGa--CAAC------AGAAGGCa-GGCGG- -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 5389 | 0.69 | 0.620321 |
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Target: 5'- uCCUGCUggugcugaagcGCUGccgGUCUUCgcccugcgCGUCgGCCa -3' miRNA: 3'- -GGACGA-----------UGACaa-CAGAAG--------GCAGgCGG- -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 8381 | 0.71 | 0.497003 |
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Target: 5'- aUCUGCgcgaUGUUGUCaucaagcUCCGaCCGCCg -3' miRNA: 3'- -GGACGaug-ACAACAGa------AGGCaGGCGG- -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 26767 | 0.73 | 0.395314 |
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Target: 5'- gCC-GCUGCUGUUG-CUgCCG-CUGCCg -3' miRNA: 3'- -GGaCGAUGACAACaGAaGGCaGGCGG- -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 2207 | 0.73 | 0.358562 |
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Target: 5'- uCCUGCUGCUGcUGcugCUccUCCGUCgguauuauCGCCg -3' miRNA: 3'- -GGACGAUGACaACa--GA--AGGCAG--------GCGG- -5' |
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| 239 | 3' | -52.3 | AC_000008.1 | + | 2106 | 1.13 | 0.000655 |
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Target: 5'- gCCUGCUACUGUUGUCUUCCGUCCGCCc -3' miRNA: 3'- -GGACGAUGACAACAGAAGGCAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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