Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 239 | 5' | -59.1 | AC_000008.1 | + | 11132 | 0.7 | 0.197002 |
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Target: 5'- aCCGcgucaGGAGGGGCGacauccgcgguugacGCGGCAGCAGa- -3' miRNA: 3'- -GGCug---CCUCCUCGU---------------CGUCGUCGUCgu -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 9736 | 0.71 | 0.160781 |
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Target: 5'- aCCGugGc--GGGCGGCAGCGgGCGGCGg -3' miRNA: 3'- -GGCugCcucCUCGUCGUCGU-CGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 3859 | 0.73 | 0.132158 |
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Target: 5'- gCgGGCGGuGGcuGCAGCGGCugaAGCGGCGg -3' miRNA: 3'- -GgCUGCCuCCu-CGUCGUCG---UCGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 2169 | 0.73 | 0.124885 |
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Target: 5'- ----aGGAGGaAGCcaGGCGGCGGCGGCAg -3' miRNA: 3'- ggcugCCUCC-UCG--UCGUCGUCGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 33197 | 0.73 | 0.11467 |
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Target: 5'- -gGGCGGuGGuGCuGCAGCAGCGcGCGa -3' miRNA: 3'- ggCUGCCuCCuCGuCGUCGUCGU-CGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 15046 | 0.76 | 0.07414 |
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Target: 5'- -gGGCGGGGGuggcgcaGGCGGCAGCAaCAGCAg -3' miRNA: 3'- ggCUGCCUCC-------UCGUCGUCGUcGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 26247 | 0.81 | 0.030618 |
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Target: 5'- uCCGugGGuGGcGGCGGCAGCuGCAGCu -3' miRNA: 3'- -GGCugCCuCC-UCGUCGUCGuCGUCGu -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 33293 | 0.84 | 0.018956 |
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Target: 5'- uCCuGCaGGAcGGAGCGGCGGCGGCAGCAg -3' miRNA: 3'- -GGcUG-CCU-CCUCGUCGUCGUCGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 26697 | 0.85 | 0.01631 |
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Target: 5'- aCCGGCGGc--AGCGGCAGCGGCAGCAa -3' miRNA: 3'- -GGCUGCCuccUCGUCGUCGUCGUCGU- -5' |
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| 239 | 5' | -59.1 | AC_000008.1 | + | 2143 | 1.1 | 0.000163 |
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Target: 5'- aCCGACGGAGGAGCAGCAGCAGCAGCAg -3' miRNA: 3'- -GGCUGCCUCCUCGUCGUCGUCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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