Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23908 | 5' | -55.2 | NC_005262.1 | + | 50443 | 0.68 | 0.668617 |
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Target: 5'- aCGUCgUCGUGCCGcgCgAGGagGACGg -3' miRNA: 3'- gGCGGaAGCACGGCaaG-UCCagCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 41683 | 0.68 | 0.657877 |
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Target: 5'- uUGCCggccaCGUGCag--CAGGUCGACGu -3' miRNA: 3'- gGCGGaa---GCACGgcaaGUCCAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 19604 | 0.69 | 0.58258 |
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Target: 5'- gUCGCCUUCgGUGCgaGUggcGGUCGGCGu -3' miRNA: 3'- -GGCGGAAG-CACGg-CAaguCCAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 2894 | 0.69 | 0.614782 |
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Target: 5'- aUCGCCUUCGaucagGCUggugagGUUCAucuGGUCGGCGc -3' miRNA: 3'- -GGCGGAAGCa----CGG------CAAGU---CCAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 20736 | 0.7 | 0.571914 |
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Target: 5'- gCUGCCgcggcgcgCgGUGCCGgcCGGGUCGAUg -3' miRNA: 3'- -GGCGGaa------G-CACGGCaaGUCCAGCUGu -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 7868 | 0.7 | 0.550731 |
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Target: 5'- gCCGCCcUCGaucGCCGgaC-GGUCGACGu -3' miRNA: 3'- -GGCGGaAGCa--CGGCaaGuCCAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 28169 | 0.7 | 0.529795 |
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Target: 5'- cCCGCCg-CGUGCCGUUgCcGGaCGACu -3' miRNA: 3'- -GGCGGaaGCACGGCAA-GuCCaGCUGu -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 29632 | 0.71 | 0.507111 |
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Target: 5'- cUCGaCCUUCGUGCCGgcgacggccggCGGGUaGACGu -3' miRNA: 3'- -GGC-GGAAGCACGGCaa---------GUCCAgCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 42560 | 1.11 | 0.001127 |
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Target: 5'- gCCGCCUUCGUGCCGUUCAGGUCGACAa -3' miRNA: 3'- -GGCGGAAGCACGGCAAGUCCAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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