Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23908 | 5' | -55.2 | NC_005262.1 | + | 8225 | 0.66 | 0.762552 |
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Target: 5'- gCGCgCUgCGaGCCGgcCGGGUCGAUg -3' miRNA: 3'- gGCG-GAaGCaCGGCaaGUCCAGCUGu -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 1069 | 0.66 | 0.762552 |
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Target: 5'- aCCGCCagCGcGCCGaUCAGGgugCGuGCGc -3' miRNA: 3'- -GGCGGaaGCaCGGCaAGUCCa--GC-UGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 2894 | 0.69 | 0.614782 |
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Target: 5'- aUCGCCUUCGaucagGCUggugagGUUCAucuGGUCGGCGc -3' miRNA: 3'- -GGCGGAAGCa----CGG------CAAGU---CCAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 50443 | 0.68 | 0.668617 |
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Target: 5'- aCGUCgUCGUGCCGcgCgAGGagGACGg -3' miRNA: 3'- gGCGGaAGCACGGCaaG-UCCagCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 34158 | 0.68 | 0.679325 |
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Target: 5'- cCCGCCggcCGUcGCCGgcacgaAGGUCGAg- -3' miRNA: 3'- -GGCGGaa-GCA-CGGCaag---UCCAGCUgu -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 30112 | 0.67 | 0.738196 |
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Target: 5'- gCCGCCgcgaagcccacgaUCGUGCCGcugCuGGUCGcCGa -3' miRNA: 3'- -GGCGGa------------AGCACGGCaa-GuCCAGCuGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 63278 | 0.67 | 0.752485 |
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Target: 5'- gCCGCCUgcUUGacgGCCGgcugcuggcUCGGcGUCGACGu -3' miRNA: 3'- -GGCGGA--AGCa--CGGCa--------AGUC-CAGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 45685 | 0.66 | 0.791942 |
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Target: 5'- gCCGCaCUUCGUGCgCaucgaGGGcCGGCAg -3' miRNA: 3'- -GGCG-GAAGCACG-Gcaag-UCCaGCUGU- -5' |
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| 23908 | 5' | -55.2 | NC_005262.1 | + | 19604 | 0.69 | 0.58258 |
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Target: 5'- gUCGCCUUCgGUGCgaGUggcGGUCGGCGu -3' miRNA: 3'- -GGCGGAAG-CACGg-CAaguCCAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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