miRNA: 3'- -CUuCAUCAGCGCCU---CGgUGUa-----CG-GCC- -5'
miRNA: 3'- -CUUCaUCAGCGCc-UCGGU--GUACGGCc -5'
miRNA: 3'- -CUUCAUCAGCGCCUCGGUGUACGGCC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23909 | 5' | -55.3 | NC_005262.1 | + | 36854 | 0.66 | 0.740149 |
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Target: 5'- gGAuGUGGUCGCGGAuagGCgGCGaauccgGCuCGGg -3' miRNA: 3'- -CUuCAUCAGCGCCU---CGgUGUa-----CG-GCC- -5' |
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| 23909 | 5' | -55.3 | NC_005262.1 | + | 22412 | 0.66 | 0.780607 |
|
Target: 5'- uGAAGcGGUCGCGuaGGUCAgcCGUGCCGu -3' miRNA: 3'- -CUUCaUCAGCGCc-UCGGU--GUACGGCc -5' |
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| 23909 | 5' | -55.3 | NC_005262.1 | + | 40982 | 1.1 | 0.001019 |
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Target: 5'- cGAAGUAGUCGCGGAGCCACAUGCCGGu -3' miRNA: 3'- -CUUCAUCAGCGCCUCGGUGUACGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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