Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23910 | 5' | -56.3 | NC_005262.1 | + | 33020 | 0.66 | 0.649782 |
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Target: 5'- cGCCAAGCUGcGCCUGaUgCUGAcGUa -3' miRNA: 3'- cUGGUUCGGC-CGGACaAgGACUuCGa -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 36180 | 0.67 | 0.605853 |
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Target: 5'- aGGCgCGAGUgcagcucgaCGGCCUGUUCgCUGucGAGCg -3' miRNA: 3'- -CUG-GUUCG---------GCCGGACAAG-GAC--UUCGa -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 15500 | 0.67 | 0.582896 |
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Target: 5'- aGCgCAGGCCGGCCucgagguUGUUCC--AAGCa -3' miRNA: 3'- cUG-GUUCGGCCGG-------ACAAGGacUUCGa -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 6756 | 0.69 | 0.498726 |
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Target: 5'- aGCCuccuuGCCGGCCaGcUCCuUGAAGCg -3' miRNA: 3'- cUGGuu---CGGCCGGaCaAGG-ACUUCGa -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 3719 | 0.69 | 0.457188 |
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Target: 5'- uGACUGAGCUgGGCCUGcgCCUGGauggguuccgcgcGGCUc -3' miRNA: 3'- -CUGGUUCGG-CCGGACaaGGACU-------------UCGA- -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 52978 | 0.74 | 0.23933 |
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Target: 5'- aGCCGGGCauggguauCGGCCUGUUCaaGggGCUg -3' miRNA: 3'- cUGGUUCG--------GCCGGACAAGgaCuuCGA- -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 53872 | 0.81 | 0.079477 |
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Target: 5'- cGCuCAAGCCGGCCgacaaggGUUCCUGAAGUc -3' miRNA: 3'- cUG-GUUCGGCCGGa------CAAGGACUUCGa -5' |
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| 23910 | 5' | -56.3 | NC_005262.1 | + | 38828 | 1.08 | 0.000883 |
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Target: 5'- cGACCAAGCCGGCCUGUUCCUGAAGCUg -3' miRNA: 3'- -CUGGUUCGGCCGGACAAGGACUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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