Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23911 | 3' | -55.3 | NC_005262.1 | + | 15787 | 0.66 | 0.76075 |
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Target: 5'- gUCUgGGUGuUGGUcGCGggGGuGCCGGGg -3' miRNA: 3'- -AGAgCCACuACUAcUGCa-CC-CGGCUC- -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 2789 | 0.67 | 0.708995 |
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Target: 5'- gUCUCGGcGAgcggcuUGAUGACGgucggcacGGCCGAc -3' miRNA: 3'- -AGAGCCaCU------ACUACUGCac------CCGGCUc -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 14134 | 0.68 | 0.622923 |
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Target: 5'- uUUUUGaGUGG-GAUGGCGUGcGCCGGGa -3' miRNA: 3'- -AGAGC-CACUaCUACUGCACcCGGCUC- -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 43305 | 0.69 | 0.612098 |
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Target: 5'- gCUCGGUGcUGAUGACGc---CCGAGg -3' miRNA: 3'- aGAGCCACuACUACUGCacccGGCUC- -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 37083 | 0.75 | 0.272767 |
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Target: 5'- gUUCGGUGAUGcgGAUGUcgaccgaucGGGCCGGc -3' miRNA: 3'- aGAGCCACUACuaCUGCA---------CCCGGCUc -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 6030 | 0.78 | 0.175567 |
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Target: 5'- cUCUCgGGUGGUGGUGugGUGcGGCaCGAu -3' miRNA: 3'- -AGAG-CCACUACUACugCAC-CCG-GCUc -5' |
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| 23911 | 3' | -55.3 | NC_005262.1 | + | 37966 | 1.09 | 0.00137 |
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Target: 5'- uUCUCGGUGAUGAUGACGUGGGCCGAGu -3' miRNA: 3'- -AGAGCCACUACUACUGCACCCGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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