Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23913 | 5' | -55 | NC_005262.1 | + | 43991 | 0.65 | 0.803118 |
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Target: 5'- aUCGcGCgGGCGauGGucgcuuaAUCGUGUCCCg -3' miRNA: 3'- cAGCaCG-CCGU--CCuug----UAGCACAGGG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 26551 | 0.65 | 0.803118 |
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Target: 5'- uUCGUGCGGUucacgcGGAAgGcgCGUGUCg- -3' miRNA: 3'- cAGCACGCCGu-----CCUUgUa-GCACAGgg -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 60255 | 0.66 | 0.793583 |
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Target: 5'- -aCG-GCGGCAagaccGGcGCAUCGUGgaugUCCg -3' miRNA: 3'- caGCaCGCCGU-----CCuUGUAGCACa---GGG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 6639 | 0.66 | 0.783884 |
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Target: 5'- -aUGUGCGGCGGcgcuucGAucGCGUCGccGUCCUc -3' miRNA: 3'- caGCACGCCGUC------CU--UGUAGCa-CAGGG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 20689 | 0.67 | 0.743659 |
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Target: 5'- uUCGUGCGGUgcAGGAAUGccUCuUG-CCCg -3' miRNA: 3'- cAGCACGCCG--UCCUUGU--AGcACaGGG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 21570 | 0.67 | 0.7333 |
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Target: 5'- aUCGgaaccGCGaGCGGGuACGUCGUGcCgCCg -3' miRNA: 3'- cAGCa----CGC-CGUCCuUGUAGCACaG-GG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 42918 | 0.72 | 0.423847 |
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Target: 5'- cGUCGUGCGGCGgcuggguaacGGAguugacugccuGCGUCGUGUagagcgggugccgcgCCCc -3' miRNA: 3'- -CAGCACGCCGU----------CCU-----------UGUAGCACA---------------GGG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 15610 | 0.73 | 0.392683 |
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Target: 5'- -gCGUGCGGCAGGAgcGCAUCGaggCgCg -3' miRNA: 3'- caGCACGCCGUCCU--UGUAGCacaGgG- -5' |
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| 23913 | 5' | -55 | NC_005262.1 | + | 37533 | 1.13 | 0.000751 |
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Target: 5'- cGUCGUGCGGCAGGAACAUCGUGUCCCa -3' miRNA: 3'- -CAGCACGCCGUCCUUGUAGCACAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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