Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23914 | 3' | -56.6 | NC_005262.1 | + | 2576 | 0.66 | 0.630457 |
Target: 5'- cGUCUCGgagaCGAGCCGGcGGCccuucauGCGGGu- -3' miRNA: 3'- -CAGAGCa---GCUUGGUC-UCGu------CGCCCuu -5' |
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23914 | 3' | -56.6 | NC_005262.1 | + | 45791 | 0.67 | 0.608604 |
Target: 5'- aGUCUCGUCGGGCCc-GGCAGCc---- -3' miRNA: 3'- -CAGAGCAGCUUGGucUCGUCGcccuu -5' |
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23914 | 3' | -56.6 | NC_005262.1 | + | 9692 | 0.69 | 0.481399 |
Target: 5'- cGUCgaCGUCGAGgCGcAGCAGCGGGu- -3' miRNA: 3'- -CAGa-GCAGCUUgGUcUCGUCGCCCuu -5' |
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23914 | 3' | -56.6 | NC_005262.1 | + | 37369 | 1.06 | 0.001316 |
Target: 5'- cGUCUCGUCGAACCAGAGCAGCGGGAAc -3' miRNA: 3'- -CAGAGCAGCUUGGUCUCGUCGCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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