Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23919 | 3' | -52.9 | NC_005262.1 | + | 41549 | 0.66 | 0.813347 |
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Target: 5'- gCGUGAU-GCGCAGgccgGCcgGCGUg-- -3' miRNA: 3'- aGUACUAgCGCGUCa---CGuaCGCGuag -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 52064 | 0.66 | 0.813347 |
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Target: 5'- ---aGAUCGCGaAGccgaGCAUGCGCAa- -3' miRNA: 3'- aguaCUAGCGCgUCa---CGUACGCGUag -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 46883 | 0.66 | 0.822825 |
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Target: 5'- uUCAUG-UgGUGCGGUGCG-GUGCGg- -3' miRNA: 3'- -AGUACuAgCGCGUCACGUaCGCGUag -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 34749 | 0.66 | 0.832093 |
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Target: 5'- aCAcGGUgCGCGUcGUGCcgGCGCcgCu -3' miRNA: 3'- aGUaCUA-GCGCGuCACGuaCGCGuaG- -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 2919 | 0.66 | 0.832093 |
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Target: 5'- uUCAucUGGUCgGCGCAGUaGUGUGUG-AUCg -3' miRNA: 3'- -AGU--ACUAG-CGCGUCA-CGUACGCgUAG- -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 20780 | 0.66 | 0.841142 |
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Target: 5'- ---aGAUCGCGCAc-GC--GCGCAUCu -3' miRNA: 3'- aguaCUAGCGCGUcaCGuaCGCGUAG- -5' |
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| 23919 | 3' | -52.9 | NC_005262.1 | + | 3321 | 0.66 | 0.841142 |
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Target: 5'- cUCGcccUGAUCGCGCAGcgucaUGUAgagcgacagGCGCAg- -3' miRNA: 3'- -AGU---ACUAGCGCGUC-----ACGUa--------CGCGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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