Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23921 | 5' | -53.2 | NC_005262.1 | + | 63064 | 0.7 | 0.634813 |
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Target: 5'- cCGUUC-CGACGCAUA-CCUgcuggcacaaGCGCUAc -3' miRNA: 3'- -GCAAGuGCUGCGUGUaGGA----------CGCGAUa -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 4528 | 0.71 | 0.557319 |
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Target: 5'- ---gCGCGACGCGCucguuucgcUCCUGCGCg-- -3' miRNA: 3'- gcaaGUGCUGCGUGu--------AGGACGCGaua -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 47895 | 0.71 | 0.524852 |
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Target: 5'- --aUCGCGGCGCACGUCgC-GCGCa-- -3' miRNA: 3'- gcaAGUGCUGCGUGUAG-GaCGCGaua -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 13452 | 0.72 | 0.48275 |
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Target: 5'- gCGgucgUCGCgGGCGCuuCGUCCUGCGCg-- -3' miRNA: 3'- -GCa---AGUG-CUGCGu-GUAGGACGCGaua -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 12657 | 0.73 | 0.442345 |
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Target: 5'- gCGcgCGCGGCGCGCGgcgaacCCUGCGCc-- -3' miRNA: 3'- -GCaaGUGCUGCGUGUa-----GGACGCGaua -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 15011 | 0.77 | 0.25249 |
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Target: 5'- uCGUgagCuCG-CGCACGUCCUGCGCUGc -3' miRNA: 3'- -GCAa--GuGCuGCGUGUAGGACGCGAUa -5' |
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| 23921 | 5' | -53.2 | NC_005262.1 | + | 32413 | 1.06 | 0.002824 |
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Target: 5'- aCGUUCACGACGCACAUCCUGCGCUAUc -3' miRNA: 3'- -GCAAGUGCUGCGUGUAGGACGCGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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