Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23934 | 3' | -61.3 | NC_005262.1 | + | 17109 | 0.66 | 0.455465 |
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Target: 5'- -cGA-GGAGGuccaCCGCGCGGCGa- -3' miRNA: 3'- cuCUcCCUCCuuagGGCGCGCCGCgu -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 60491 | 0.66 | 0.446052 |
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Target: 5'- --cAGGGcGaGAcgCcgggCCGCGCGGCGCAg -3' miRNA: 3'- cucUCCCuC-CUuaG----GGCGCGCCGCGU- -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 22368 | 0.66 | 0.427559 |
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Target: 5'- uGGuGcGGAGGGuGUCCgGCGCGGauCGCGa -3' miRNA: 3'- cUCuC-CCUCCU-UAGGgCGCGCC--GCGU- -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 61641 | 0.66 | 0.418484 |
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Target: 5'- cGAGGGcgaGGAGGaAGUgCUGUaccGCGGCGCGc -3' miRNA: 3'- -CUCUC---CCUCC-UUAgGGCG---CGCCGCGU- -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 15570 | 0.69 | 0.304586 |
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Target: 5'- cGAGAGcaGGAGGAG-CUggaggCGCGCGcGCGCGa -3' miRNA: 3'- -CUCUC--CCUCCUUaGG-----GCGCGC-CGCGU- -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 24926 | 0.69 | 0.303864 |
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Target: 5'- cGAGGcauGGcGGGAugcgcacAUgCCGCGCGGCGCGc -3' miRNA: 3'- -CUCU---CCcUCCU-------UAgGGCGCGCCGCGU- -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 1767 | 0.72 | 0.190197 |
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Target: 5'- cGAGGaGGAGG-AUCgCGCGCGGCGa- -3' miRNA: 3'- -CUCUcCCUCCuUAGgGCGCGCCGCgu -5' |
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| 23934 | 3' | -61.3 | NC_005262.1 | + | 23858 | 1.07 | 0.000402 |
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Target: 5'- aGAGAGGGAGGAAUCCCGCGCGGCGCAc -3' miRNA: 3'- -CUCUCCCUCCUUAGGGCGCGCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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