Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23935 | 5' | -54.4 | NC_005262.1 | + | 45348 | 0.69 | 0.627884 |
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Target: 5'- gGCGGCGCGGGCGUCcUgaaccagcaauacaGCaccUCGGGu -3' miRNA: 3'- -CGUCGCGCUCGCAGcA--------------UGaa-AGCCCu -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 33394 | 0.7 | 0.598532 |
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Target: 5'- cCAGCGCacGCGgcUCGUGCUguacUCGGGGu -3' miRNA: 3'- cGUCGCGcuCGC--AGCAUGAa---AGCCCU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 36822 | 0.7 | 0.598532 |
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Target: 5'- -gGGCGUGAGCGaugccauuucuUCGU-CggUCGGGAu -3' miRNA: 3'- cgUCGCGCUCGC-----------AGCAuGaaAGCCCU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 17221 | 0.71 | 0.513354 |
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Target: 5'- aCGGCGCuGGCGUCGUggucgucguggGCgucgCGGGAg -3' miRNA: 3'- cGUCGCGcUCGCAGCA-----------UGaaa-GCCCU- -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 26848 | 0.77 | 0.263088 |
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Target: 5'- aGCGGCGCGAGCGUgGUGaucUCGGc- -3' miRNA: 3'- -CGUCGCGCUCGCAgCAUgaaAGCCcu -5' |
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| 23935 | 5' | -54.4 | NC_005262.1 | + | 23666 | 1.11 | 0.001241 |
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Target: 5'- uGCAGCGCGAGCGUCGUACUUUCGGGAu -3' miRNA: 3'- -CGUCGCGCUCGCAGCAUGAAAGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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