Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23953 | 5' | -60 | NC_005262.1 | + | 49176 | 0.66 | 0.522525 |
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Target: 5'- ---gGCCGcAGCG-GCgCGcacggcgaugaUCUCCGGCa -3' miRNA: 3'- ccuaCGGC-UCGCaCGgGC-----------AGAGGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 4878 | 0.66 | 0.532587 |
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Target: 5'- --uUGCCGAGCGccgacucGaCCUG-CUUCGGCg -3' miRNA: 3'- ccuACGGCUCGCa------C-GGGCaGAGGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 52207 | 0.66 | 0.532587 |
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Target: 5'- uGGAagucGCuCGAGCGcUGgCCGcCgCCGGCa -3' miRNA: 3'- -CCUa---CG-GCUCGC-ACgGGCaGaGGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 55634 | 0.66 | 0.542718 |
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Target: 5'- aGAaGCCGGGCcgcgcgcucGUGgCCGcgUCCGGCa -3' miRNA: 3'- cCUaCGGCUCG---------CACgGGCagAGGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 10400 | 0.66 | 0.542718 |
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Target: 5'- -cGUGCCG-GCGuUGUCCuugaacgcgGUCUgCGGCa -3' miRNA: 3'- ccUACGGCuCGC-ACGGG---------CAGAgGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 59696 | 0.66 | 0.552911 |
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Target: 5'- cGGcGUGCCGccacGCGggcGCUaCGUCgCCGGCa -3' miRNA: 3'- -CC-UACGGCu---CGCa--CGG-GCAGaGGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 6372 | 0.66 | 0.552911 |
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Target: 5'- cGGUGCCgGAGCGUugucGgCCGcCUucUCGGCg -3' miRNA: 3'- cCUACGG-CUCGCA----CgGGCaGA--GGCCG- -5' |
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| 23953 | 5' | -60 | NC_005262.1 | + | 12793 | 0.66 | 0.552911 |
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Target: 5'- cGGUcGCCGgucgcuuccuuGGCcUGCUCGaccuUCUCCGGCg -3' miRNA: 3'- cCUA-CGGC-----------UCGcACGGGC----AGAGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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