Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23963 | 5' | -56.9 | NC_005262.1 | + | 3830 | 0.67 | 0.605155 |
Target: 5'- ---aAGGUGCCGAGcgCC-CCGCGcGUg -3' miRNA: 3'- cacaUCCGCGGCUUaaGGaGGCGC-CA- -5' |
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23963 | 5' | -56.9 | NC_005262.1 | + | 13793 | 0.68 | 0.551655 |
Target: 5'- -gGUGGGCGCCGGcgcggcUUCaUCgGCGGg -3' miRNA: 3'- caCAUCCGCGGCUu-----AAGgAGgCGCCa -5' |
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23963 | 5' | -56.9 | NC_005262.1 | + | 54637 | 0.69 | 0.479488 |
Target: 5'- cGUGaAGGCGCCGggUga-UCCGCa-- -3' miRNA: 3'- -CACaUCCGCGGCuuAaggAGGCGcca -5' |
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23963 | 5' | -56.9 | NC_005262.1 | + | 18299 | 0.7 | 0.412255 |
Target: 5'- -gGUAGGCGCgCcGGUUCgCUUCGCGGc -3' miRNA: 3'- caCAUCCGCG-GcUUAAG-GAGGCGCCa -5' |
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23963 | 5' | -56.9 | NC_005262.1 | + | 5062 | 1.07 | 0.001171 |
Target: 5'- cGUGUAGGCGCCGAAUUCCUCCGCGGUc -3' miRNA: 3'- -CACAUCCGCGGCUUAAGGAGGCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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