Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23966 | 5' | -48.1 | NC_005262.1 | + | 61143 | 0.71 | 0.846678 |
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Target: 5'- cGCGCUG--GCGCAGCAG-AUCgCGGc -3' miRNA: 3'- cCGCGAUaaUGUGUUGUCuUAG-GCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 48648 | 0.72 | 0.814836 |
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Target: 5'- aGGUGCUG--ACGCAGCAGGAgcucgacaagcugCCGGc -3' miRNA: 3'- -CCGCGAUaaUGUGUUGUCUUa------------GGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 10971 | 0.72 | 0.798991 |
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Target: 5'- cGCGCUGUcgGCGCGcCGGGAacgCCGGUc -3' miRNA: 3'- cCGCGAUAa-UGUGUuGUCUUa--GGCCA- -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 7317 | 0.73 | 0.788838 |
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Target: 5'- aGGCGCUucUGCACGuu-GAcgCCGGUu -3' miRNA: 3'- -CCGCGAuaAUGUGUuguCUuaGGCCA- -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 9604 | 0.73 | 0.787813 |
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Target: 5'- gGGCGCUGgaGCACGcgcucggACAGGA-CCGGc -3' miRNA: 3'- -CCGCGAUaaUGUGU-------UGUCUUaGGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 35458 | 0.74 | 0.724641 |
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Target: 5'- aGGCGCUGcacGCGC-ACGGGcAUCCGGa -3' miRNA: 3'- -CCGCGAUaa-UGUGuUGUCU-UAGGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 17113 | 0.75 | 0.691016 |
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Target: 5'- cGGCGCUGacUGCAuCGGCGGGcgUCGGUg -3' miRNA: 3'- -CCGCGAUa-AUGU-GUUGUCUuaGGCCA- -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 1412 | 1.11 | 0.004687 |
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Target: 5'- gGGCGCUAUUACACAACAGAAUCCGGUg -3' miRNA: 3'- -CCGCGAUAAUGUGUUGUCUUAGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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