Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23977 | 5' | -58.8 | NC_005262.1 | + | 34577 | 0.66 | 0.552653 |
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Target: 5'- -aGUCGCGCGCCGccgCGcGGAuGUGCUc -3' miRNA: 3'- uaCGGCGCGUGGUa--GC-CCUcCAUGAc -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 20490 | 0.67 | 0.499838 |
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Target: 5'- aGUGCguaaucgCGCGCACCAUCGGcGcGGcGCUc -3' miRNA: 3'- -UACG-------GCGCGUGGUAGCC-CuCCaUGAc -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 1492 | 0.67 | 0.480727 |
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Target: 5'- -cGCUGcCGCugCAUCuGcGGAGGgagGCUGc -3' miRNA: 3'- uaCGGC-GCGugGUAG-C-CCUCCa--UGAC- -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 41880 | 0.67 | 0.461004 |
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Target: 5'- aAUGCCaGCGCAUaguaGGGGGGU-CUGu -3' miRNA: 3'- -UACGG-CGCGUGguagCCCUCCAuGAC- -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 26127 | 0.68 | 0.441717 |
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Target: 5'- cUGCuUGCGCGCCG-CGGcGAGcUGCUGg -3' miRNA: 3'- uACG-GCGCGUGGUaGCC-CUCcAUGAC- -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 36017 | 0.69 | 0.390262 |
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Target: 5'- aGUGCCGuCGCAUCAUCGugccgcguGGAgacgaccguacgcccGGUACUGc -3' miRNA: 3'- -UACGGC-GCGUGGUAGC--------CCU---------------CCAUGAC- -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 53637 | 0.69 | 0.386739 |
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Target: 5'- -cGCCGaGCACCAUCucGGGGUACg- -3' miRNA: 3'- uaCGGCgCGUGGUAGccCUCCAUGac -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 60083 | 0.71 | 0.257117 |
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Target: 5'- -cGCCGCGCAUCAucUCGaGGAucgGGUGCa- -3' miRNA: 3'- uaCGGCGCGUGGU--AGC-CCU---CCAUGac -5' |
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| 23977 | 5' | -58.8 | NC_005262.1 | + | 5658 | 1.05 | 0.000875 |
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Target: 5'- gAUGCCGCGCACCAUCGGGAGGUACUGc -3' miRNA: 3'- -UACGGCGCGUGGUAGCCCUCCAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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