Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23978 | 5' | -50.4 | NC_005262.1 | + | 46851 | 0.67 | 0.932757 |
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Target: 5'- cGCGCGuc-GCcgccUGUAcGACCGGcGUGg -3' miRNA: 3'- -CGCGCuaaCGua--ACAUuCUGGCC-CAC- -5' |
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| 23978 | 5' | -50.4 | NC_005262.1 | + | 20852 | 0.67 | 0.92705 |
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Target: 5'- uGCGCGAUcucgugGCGcugggGUucGACCGGGc- -3' miRNA: 3'- -CGCGCUAa-----CGUaa---CAuuCUGGCCCac -5' |
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| 23978 | 5' | -50.4 | NC_005262.1 | + | 24233 | 0.68 | 0.90133 |
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Target: 5'- cGC-CGAUUGguUgaGUAAGAUCGGGa- -3' miRNA: 3'- -CGcGCUAACguAa-CAUUCUGGCCCac -5' |
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| 23978 | 5' | -50.4 | NC_005262.1 | + | 7253 | 0.69 | 0.862892 |
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Target: 5'- aCGCGGUagcgGCAUgc---GGCCGGGUGu -3' miRNA: 3'- cGCGCUAa---CGUAacauuCUGGCCCAC- -5' |
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| 23978 | 5' | -50.4 | NC_005262.1 | + | 12999 | 0.73 | 0.647978 |
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Target: 5'- cGCGCaGGUUGUcgUGUccagcAGGCCGGGg- -3' miRNA: 3'- -CGCG-CUAACGuaACAu----UCUGGCCCac -5' |
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| 23978 | 5' | -50.4 | NC_005262.1 | + | 7197 | 1.11 | 0.002994 |
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Target: 5'- gGCGCGAUUGCAUUGUAAGACCGGGUGu -3' miRNA: 3'- -CGCGCUAACGUAACAUUCUGGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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