Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23986 | 5' | -53.1 | NC_005262.1 | + | 57949 | 0.66 | 0.81492 |
Target: 5'- -aACGUGGaGGGCGcGAgCGCGCUUg -3' miRNA: 3'- uaUGCGUCaCUCGCuUUgGCGCGAAg -5' |
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23986 | 5' | -53.1 | NC_005262.1 | + | 59264 | 0.77 | 0.255952 |
Target: 5'- -cGCGCAG-GAGCGAAACgaGCGCgUCg -3' miRNA: 3'- uaUGCGUCaCUCGCUUUGg-CGCGaAG- -5' |
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23986 | 5' | -53.1 | NC_005262.1 | + | 60482 | 0.67 | 0.784441 |
Target: 5'- cUGCGCGaucaGGGCGAGacgccggGCCGCGCggCg -3' miRNA: 3'- uAUGCGUca--CUCGCUU-------UGGCGCGaaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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