Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23991 | 3' | -56.2 | NC_005262.1 | + | 52898 | 0.66 | 0.669781 |
Target: 5'- uCGCCGAGcgccugccacUGCGaauccuUGAAGC-GGCCGUAGc -3' miRNA: 3'- -GUGGCUC----------AUGC------ACUUCGuCCGGCGUU- -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 25732 | 0.66 | 0.691571 |
Target: 5'- gGCCGAG-GCGaucgcGCAGGCCGgCAAg -3' miRNA: 3'- gUGGCUCaUGCacuu-CGUCCGGC-GUU- -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 15597 | 0.66 | 0.680699 |
Target: 5'- gCGCgCGAGgagcGCGUGcGGCAGGagCGCAu -3' miRNA: 3'- -GUG-GCUCa---UGCACuUCGUCCg-GCGUu -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 23356 | 0.66 | 0.636856 |
Target: 5'- gGCCG---GCGUGAAGCGcgcGGCgGCGAa -3' miRNA: 3'- gUGGCucaUGCACUUCGU---CCGgCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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