Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23991 | 3' | -56.2 | NC_005262.1 | + | 6228 | 0.7 | 0.408306 |
Target: 5'- cCGCCGAGaUGC-UGAAGCccGCCGCGc -3' miRNA: 3'- -GUGGCUC-AUGcACUUCGucCGGCGUu -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 4723 | 0.67 | 0.592938 |
Target: 5'- --gCGGGUugGUG-AGCAGGCgGUg- -3' miRNA: 3'- gugGCUCAugCACuUCGUCCGgCGuu -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 3834 | 0.67 | 0.603891 |
Target: 5'- uGCCGAGcgccccgcGCGUGAcgugccgcugauGGUAGGCCGaCAGu -3' miRNA: 3'- gUGGCUCa-------UGCACU------------UCGUCCGGC-GUU- -5' |
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23991 | 3' | -56.2 | NC_005262.1 | + | 768 | 0.66 | 0.658827 |
Target: 5'- uCGCCGAGgugACGUGc-GCGGaCUGCAAg -3' miRNA: 3'- -GUGGCUCa--UGCACuuCGUCcGGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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