Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 240 | 5' | -55 | AC_000008.1 | + | 10503 | 0.66 | 0.644858 |
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Target: 5'- aCCGCcGC-CGGcaUCACCUGgauguccagguacaUCUACGGa -3' miRNA: 3'- -GGUGuCGaGCC--AGUGGACg-------------AGAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 18991 | 0.67 | 0.573121 |
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Target: 5'- aCCACAGaC-CGGUC-CCaGCguuugacgCUGCGGu -3' miRNA: 3'- -GGUGUC-GaGCCAGuGGaCGa-------GAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 13712 | 0.67 | 0.57201 |
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Target: 5'- gCCGCAGCgccuagaUCGGaCAagCUGCUuggcCUGCGGa -3' miRNA: 3'- -GGUGUCG-------AGCCaGUg-GACGA----GAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 6999 | 0.7 | 0.42974 |
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Target: 5'- gCGCAGCUuguugaccagcucggCGGUgACCUGCacgUCUAgGGc -3' miRNA: 3'- gGUGUCGA---------------GCCAgUGGACG---AGAUgCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 18381 | 0.7 | 0.416161 |
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Target: 5'- uCCACGGC-CGGUgGaggCUcCUCUACGGg -3' miRNA: 3'- -GGUGUCGaGCCAgUg--GAcGAGAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 17271 | 0.73 | 0.280309 |
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Target: 5'- gCCGCGGUgcaggCGGUCGCUgcgGCcgcguccaagaccUCUACGGa -3' miRNA: 3'- -GGUGUCGa----GCCAGUGGa--CG-------------AGAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 2541 | 0.79 | 0.113343 |
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Target: 5'- gCUGCAGCcaguaagUGGUCAgCUGCUCUAUGGa -3' miRNA: 3'- -GGUGUCGa------GCCAGUgGACGAGAUGCC- -5' |
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| 240 | 5' | -55 | AC_000008.1 | + | 5171 | 1.12 | 0.000461 |
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Target: 5'- cCCACAGCUCGGUCACCUGCUCUACGGc -3' miRNA: 3'- -GGUGUCGAGCCAGUGGACGAGAUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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